Structure of PDB 1b8s Chain A

Receptor sequence
>1b8sA (length=498) Species: 1931 (Streptomyces sp.) [Search protein sequence]
GYVPAVVIGTGYGAAVSALRLGEAGVQTLMLEMGQLWNQPGPDGNIFCGM
LNPDKRSSWFKNRTEAPLGSFLWLDVVNRNIDPYAGVLDRVNYDQMSVYV
GRGVGGGSLVNGGMAVEPKRSYFEEILPRVDSSEMYDRYFPRANSMLRVN
HIDTKWFEDTEWYKFARVSREQAGKAGLGTVFVPNVYDFGYMQREAAGEV
PKSALATEVIYGNNHGKQSLDKTYLAAALGTGKVTIQTLHQVKTIRQTKD
GGYALTVEQKDTDGKLLATKEISCRYLFLGAGSLGSTELLVRARDTGTLP
NLNSEVGAGWGPNGNIMTARANHMWNPTGAHQSSIPALGIDAWDNSDSSV
FAQIAPMPAGLETWVSLYLAITKNPQRGTFVYDAATDRAKLNWTRDQNAP
AVNAAKALFDRINKANGTIYRYDLFGTQLKAFADDFCYHPLGGCVLGKAT
DDYGRVAGYKNLYVTDGSLIPGSVGVNPFVTITALAERNVERIIKQDV
3D structure
PDB1b8s Crystal structure determination of cholesterol oxidase from Streptomyces and structural characterization of key active site mutants.
ChainA
Resolution1.65 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Q361 H447 N485
Catalytic site (residue number reindexed from 1) Q353 H439 N477
Enzyme Commision number 1.1.3.6: cholesterol oxidase.
5.3.3.1: steroid Delta-isomerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAD A I16 G17 G19 Y20 L39 E40 M41 Y107 R110 G114 G115 N119 G120 M122 Q249 V250 A289 G290 Y446 H447 D474 N485 P486 F487 I8 G9 G11 Y12 L31 E32 M33 Y99 R102 G106 G107 N111 G112 M114 Q241 V242 A281 G282 Y438 H439 D466 N477 P478 F479
Gene Ontology
Molecular Function
GO:0004769 steroid delta-isomerase activity
GO:0016491 oxidoreductase activity
GO:0016614 oxidoreductase activity, acting on CH-OH group of donors
GO:0016853 isomerase activity
GO:0016995 cholesterol oxidase activity
GO:0050660 flavin adenine dinucleotide binding
Biological Process
GO:0006707 cholesterol catabolic process
GO:0008203 cholesterol metabolic process
Cellular Component
GO:0005576 extracellular region

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Biological Process
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Cellular Component
External links
PDB RCSB:1b8s, PDBe:1b8s, PDBj:1b8s
PDBsum1b8s
PubMed10194345
UniProtP12676|CHOD_STRS0 Cholesterol oxidase (Gene Name=choA)

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