Structure of PDB 1b0j Chain A

Receptor sequence

>1b0jA (length=753) Species: 9823 (Sus scrofa)

RAKVAMSHFEPHEYIRYDLLEKNIDIVRKRLNRPLTLSEKIVYGHLDDPA
NQEIERGKTYLRLRPDRVAMQDATAQMAMLQFISSGLPKVAVPSTIHCDH
LIEAQLGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWRPGSGIIHQIIL
ENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPK
VIGVKLTGSLSGWTSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCT
GMATICNMGAEIGATTSVFPYNHRMKKYLSKTGRADIANLADEFKDHLVP
DPGCHYDQVIEINLSELKPHINGPFTPDLAHPVAEVGSVAEKEGWPLDIR
VGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIE
RDGYAQVLRDVGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNFTG
RNDANPETHAFVTSPEIVTALAIAGTLKFNPETDFLTGKDGKKFKLEAPD
ADELPRAEFDPGQDTYQHPPKDSSGQRVDVSPTSQRLQLLEPFDKWDGKD
LEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENRK
ANSVRNAVTQEFGPVPDTARYYKQHGIRWVVIGDENYGEGASREHSALEP
RHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHPVDKLTIQG
LKDFAPGKPLKCIIKHPNGTQETILLNHTFNETQIEWFRAGSALNRMKEL
QQK

3D structure

• PDB: 1b0j The mechanism of aconitase: 1.8 A resolution crystal structure of the S642a:citrate complex
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D100 H101 D165 R447 A642 R644
• Catalytic site (residue number reindexed from 1): D99 H100 D164 R446 A641 R643
• Enzyme Commision number: 4.2.1.3: aconitate hydratase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	A	H101 H167 S357 C358 C421 C424 I425 N446	H100 H166 S356 C357 C420 C423 I424 N445

Gene Ontology

Molecular Function

• GO:0003994 aconitate hydratase activity
• GO:0016829 lyase activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006099 tricarboxylic acid cycle

Cellular Component

• GO:0005739 mitochondrion
• GO:0005829 cytosol

External links

• PDB: RCSB:1b0j, PDBe:1b0j, PDBj:1b0j
• PDBsum: 1b0j
• PubMed: 10631981
• UniProt: P16276|ACON_PIG Aconitate hydratase, mitochondrial (Gene Name=ACO2)