Structure of PDB 1az0 Chain A

Receptor sequence
>1az0A (length=237) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENE
KIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVKTYNINELNE
IPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDS
EDEFLDYWRNYERTSQLRNDKYNNISEYRNWIYRGRK
3D structure
PDB1az0 Conformational transitions and structural deformability of EcoRV endonuclease revealed by crystallographic analysis.
ChainA
Resolution2.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A T37 N70 K92 T93 T94 Y95 T106 G109 T111 S112 K119 N120 S183 T186 N188 T36 N69 K91 T92 T93 Y94 T105 G108 T110 S111 K118 N119 S175 T178 N180
BS02 dna A Q69 S183 G184 N185 S223 Q68 S175 G176 N177 S215
BS03 CA A D74 D90 D73 D89
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1az0, PDBe:1az0, PDBj:1az0
PDBsum1az0
PubMed9367757
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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