Structure of PDB 1aqj Chain A

Receptor sequence

>1aqjA (length=381) Species: 271 (Thermus aquaticus)

VETPPEVVDFMVSLAEAPRGGRVLEPACAHGPFLRAFREAHGTGYRFVGV
EIDPKALDLPPWAEGILADFLLWEPGEAFDLILGNPPYGIVAVKDLYKKA
FSTWKGKYNLYGAFLEKAVRLLKPGGVLVFVVPATWLVLEDFALLREFLA
REGKTSVYYLGEVFPQKKVSAVVIRFQKSGKGLSLWDTQESESGFTPILW
AEYPHWEGEIIRFETEETRKLEISGMPLGDLFHIRFAARSPEFKKHPAVR
KEPGPGLVPVLTGRNLKPGWVDYEKNHSGLWMPKERAKELRDFYATPHLV
VAHTKGTRVVAAWDERAYPWREEFHLLPKEGVRLDPSSLVQWLNSEAMQK
HVRTLYRDFVPHLTLRMLERLPVRREYGFHT

3D structure

• PDB: 1aqj Differential binding of S-adenosylmethionine S-adenosylhomocysteine and Sinefungin to the adenine-specific DNA methyltransferase M.TaqI.
• Chain: A
• Resolution: 2.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N105 P106 Y108 F196
• Catalytic site (residue number reindexed from 1): N85 P86 Y88 F164
• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SFG	A	V21 A47 E71 F90 N105 P106 P107 Y108 F146	V1 A27 E51 F70 N85 P86 P87 Y88 F114	PDBbind-CN: -logKd/Ki=6.47,Kd=0.34uM

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0008168 methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0006304 DNA modification
• GO:0009307 DNA restriction-modification system
• GO:0032259 methylation

External links

• PDB: RCSB:1aqj, PDBe:1aqj, PDBj:1aqj
• PDBsum: 1aqj
• PubMed: 8995524
• UniProt: P14385|MTTA_THEAQ Type II methyltransferase M.TaqI (Gene Name=taqIM)