Structure of PDB 1ao8 Chain A

Receptor sequence
>1ao8A (length=162) Species: 1582 (Lacticaseibacillus casei) [Search protein sequence]
TAFLWAQDRDGLIGKDGHLPWHLPDDLHYFRAQTVGKIMVVGRRTYESFP
KRPLPERTNVVLTHQEDYQAQGAVVVHDVAAVFAYAKQHPDQELVIAGGA
QIFTAFKDDVDTLLVTRLAGSFEGDTKMIPLNWDDFTKVSSRTVEDTNPA
LTHTYEVWQKKA
3D structure
PDB1ao8 The solution structure of the complex of Lactobacillus casei dihydrofolate reductase with methotrexate.
ChainA
ResolutionN/A
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) L4 L19 W21 D26 L27 F30 L54 L94 T116
Catalytic site (residue number reindexed from 1) L4 L19 W21 D26 L27 F30 L54 L94 T116
Enzyme Commision number 1.5.1.3: dihydrofolate reductase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MTX A L4 A6 D26 L27 F30 R31 F49 R57 L4 A6 D26 L27 F30 R31 F49 R57 PDBbind-CN: -logKd/Ki=8.22,IC50=6nM
BindingDB: IC50=16nM,Ki=0.004000nM
Gene Ontology
Molecular Function
GO:0004146 dihydrofolate reductase activity
GO:0016491 oxidoreductase activity
GO:0050661 NADP binding
Biological Process
GO:0006730 one-carbon metabolic process
GO:0031427 response to methotrexate
GO:0046452 dihydrofolate metabolic process
GO:0046654 tetrahydrofolate biosynthetic process
GO:0046655 folic acid metabolic process
GO:0046677 response to antibiotic
Cellular Component
GO:0005829 cytosol

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External links
PDB RCSB:1ao8, PDBe:1ao8, PDBj:1ao8
PDBsum1ao8
PubMed9514736
UniProtP00381|DYR_LACCA Dihydrofolate reductase (Gene Name=folA)

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