Structure of PDB 1amu Chain A

Receptor sequence
>1amuA (length=509) Species: 1393 (Brevibacillus brevis) [Search protein sequence]
GTHEEEQYLFAVNNTKAEYPRDKTIHQLFEEQVSKRPNNVAIVCENEQLT
YHELNVKANQLARIFIEKGIGKDTLVGIMMEKSIDLFIGILAVLKAGGAY
VPIDIEYPKERIQYILDDSQARMLLTQKHLVHLIHNIQFNGQVEIFEEDT
IKIREGTNLHVPSKSTDLAYVIYTSPKGTMLEHKGISNLKVFFENSLNVT
EKDRIGQFASISFDASVWEMFMALLTGASLYIILKDTINDFVKFEQYINQ
KEITVITLPPTYVVHLDPERILSIQTLITAGSATSPSLVNKWKEKVTYIN
AYGPTETTICATTWVATKETIGHSVPIGAPIQNTQIYIVDENLQLKSVGE
AGELCIGGEGLARGYWKRPELTSQKFVDNPFVPGEKLYKTGDQARWLSDG
NIEYLGRIDNQVKIRGHRVELEEVESILLKHMYISETAVSVHKDHQEQPY
LCAYFVSEKHIPLEQLRQFSSEELPTYMIPSYFIQLDKMPLTSNGKIDRK
QLPEPDLTF
3D structure
PDB1amu Structural basis for the activation of phenylalanine in the non-ribosomal biosynthesis of gramicidin S.
ChainA
Resolution1.9 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) T190 L210 T326 E327 K434 R439 K517
Catalytic site (residue number reindexed from 1) T174 L189 T305 E306 K413 R418 K496
Enzyme Commision number 5.1.1.11: phenylalanine racemase (ATP-hydrolyzing).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 PHE A F234 D235 W239 A301 G324 I330 K517 F213 D214 W218 A280 G303 I309 K496
BS02 AMP A T190 G302 S303 A304 A322 Y323 T326 D413 Y425 K517 T174 G281 S282 A283 A301 Y302 T305 D392 Y404 K496
External links
PDB RCSB:1amu, PDBe:1amu, PDBj:1amu
PDBsum1amu
PubMed9250661
UniProtP0C061|GRSA_ANEMI Gramicidin S synthase 1 (Gene Name=grsA)

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