Structure of PDB 1aj6 Chain A

Receptor sequence
>1aj6A (length=194) Species: 562 (Escherichia coli) [Search protein sequence]
VLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEIIV
TIHADNSVSVQDDGRGIPTGIVSAAEVIMTVLHAGGKFSGGLHGVGVSVV
NALSQKLELVIQHEGKIHRQIYEHGVPQAPLAVTGETEKTGTMVRFWPSL
ETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFH
3D structure
PDB1aj6 The entropic penalty of ordered water accounts for weaker binding of the antibiotic novobiocin to a resistant mutant of DNA gyrase: a thermodynamic and crystallographic study.
ChainA
Resolution2.3 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NOV A N46 R76 I78 P79 I94 N35 R65 I67 P68 I78 MOAD: Kd=1.2uM
PDBbind-CN: -logKd/Ki=5.92,Kd=1.2uM
BindingDB: IC50=170nM,Kd=7.0nM,Ki=7.0nM
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
GO:0005524 ATP binding
Biological Process
GO:0006265 DNA topological change

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Molecular Function
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Biological Process
External links
PDB RCSB:1aj6, PDBe:1aj6, PDBj:1aj6
PDBsum1aj6
PubMed9245398
UniProtP0AES6|GYRB_ECOLI DNA gyrase subunit B (Gene Name=gyrB)

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