Structure of PDB 1afs Chain A

Receptor sequence
>1afsA (length=319) Species: 10116 (Rattus norvegicus) [Search protein sequence]
MDSISLRVALNDGNFIPVLGFGTTVPEKVAKDEVIKATKIAIDNGFRHFD
SAYLYEVEEEVGQAIRSKIEDGTVKREDIFYTSKLWSTFHRPELVRTCLE
KTLKSTQLDYVDLYIIHFPMALQPGDIFFPRDEHGKLLFETVDICDTWEA
MEKCKDAGLAKSIGVSNFNCRQLERILNKPGLKYKPVCNQVECHLYLNQS
KMLDYCKSKDIILVSYCTLGSSRDKTWVDQKSPVLLDDPVLCAIAKKYKQ
TPALVALRYQLQRGVVPLIRSFNAKRIKELTQVFEFQLASEDMKALDGLN
RNFRYNNAKYFDDHPNHPF
3D structure
PDB1afs Steroid recognition and regulation of hormone action: crystal structure of testosterone and NADP+ bound to 3 alpha-hydroxysteroid/dihydrodiol dehydrogenase.
ChainA
Resolution2.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D50 Y55 K84 H117
Catalytic site (residue number reindexed from 1) D50 Y55 K84 H117
Enzyme Commision number 1.1.1.50: 3alpha-hydroxysteroid 3-dehydrogenase (Si-specific).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NAP A G22 T24 D50 Y55 H117 N167 Q190 Y216 C217 L219 S221 S222 A253 R270 S271 R276 E279 L280 G22 T24 D50 Y55 H117 N167 Q190 Y216 C217 L219 S221 S222 A253 R270 S271 R276 E279 L280
BS02 TES A Y55 H117 W227 Y310 Y55 H117 W227 Y310
Gene Ontology
Molecular Function
GO:0004032 aldose reductase (NADPH) activity
GO:0016229 steroid dehydrogenase activity
GO:0016491 oxidoreductase activity
GO:0032052 bile acid binding
GO:0047023 androsterone dehydrogenase activity
GO:0047042 androsterone dehydrogenase (B-specific) activity
GO:0047086 ketosteroid monooxygenase activity
Biological Process
GO:0006693 prostaglandin metabolic process
GO:0008202 steroid metabolic process
GO:0021766 hippocampus development
GO:0042448 progesterone metabolic process
GO:0044597 daunorubicin metabolic process
GO:0044598 doxorubicin metabolic process
Cellular Component
GO:0005737 cytoplasm
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1afs, PDBe:1afs, PDBj:1afs
PDBsum1afs
PubMed9261071
UniProtP23457|DIDH_RAT 3-alpha-hydroxysteroid dehydrogenase (Gene Name=Akr1c9)

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