Structure of PDB 1af7 Chain A

Receptor sequence
>1af7A (length=274) [Search protein sequence]
SVLLQMTQRLALSDAHFRRICQLIYQRAGIVLADHKRDMVYNRLVRRLRA
LGLDDFGRYLSMLEANQNSAEWQAFINALTTNLTAFFREAHHFPILAEHA
RRRHGEYRVWSAAASTGEEPYSIAITLADALGMAPGRWKVFASDIDTEVL
EKARSGIYRLSELKTLSPQQLQRYFMRGTGPHEGLVRVRQELANYVEFSS
VNLLEKQYNVPGPFDAIFCRNVMIYFDKTTQEDILRRFVPLLKPDGLLFA
GHSENFSNLVREFSLRGQTVYALS
3D structure
PDB1af7 Crystal structure of the chemotaxis receptor methyltransferase CheR suggests a conserved structural motif for binding S-adenosylmethionine.
ChainA
Resolution2.0 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) R53 D154
Catalytic site (residue number reindexed from 1) R43 D144
Enzyme Commision number 2.1.1.80: protein-glutamate O-methyltransferase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SAH A T91 N92 T94 R98 A123 E129 D154 I155 N212 L213 R230 N231 V232 F236 T81 N82 T84 R88 A113 E119 D144 I145 N202 L203 R220 N221 V222 F226
Gene Ontology
Molecular Function
GO:0008168 methyltransferase activity
GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
GO:0008983 protein-glutamate O-methyltransferase activity
Biological Process
GO:0006935 chemotaxis
GO:0032259 methylation

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Molecular Function
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Biological Process
External links
PDB RCSB:1af7, PDBe:1af7, PDBj:1af7
PDBsum1af7
PubMed9115443
UniProtP07801|CHER_SALTY Chemotaxis protein methyltransferase (Gene Name=cheR)

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