Structure of PDB 1a35 Chain A

Receptor sequence

>1a35A (length=474) Species: 9606 (Homo sapiens)

IKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEEVATFFAKML
DHEYTTKEIFRKNFFKDWRKEMTNEEKNIITNLSKCDFTQMSQYFKAQTE
ARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIANFKIEPPGLFRGR
GNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLV
SWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYRED
WKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINL
HPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDL
FDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENIPA
KILSYNRANRAVAILCNHQRAQIALGTSKLNFLDPRITVAWCKKWGVPIE
KIYNKTQREKFAWAIDMADEDYEF

3D structure

• PDB: 1a35 Crystal structures of human topoisomerase I in covalent and noncovalent complexes with DNA.
• Chain: A
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 5.6.2.1: DNA topoisomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	R364 T411 W412 I424 Y426 M428 K439 K443 R488 H632 Q633 R634 A635 T718 F723	R150 T197 W198 I210 Y212 M214 K225 K229 R274 H418 Q419 R420 A421 T427 F432
BS02	dna	A	K354 E356 F361 R362 G363 R364 H367 R488 A489 G490 N491 K493 T501 K532 N574 T585 A586 K587 T747	K140 E142 F147 R148 G149 R150 H153 R274 A275 G276 N277 K279 T287 K318 N360 T371 A372 K373 T456

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity

Biological Process

• GO:0006265 DNA topological change

Cellular Component

• GO:0005694 chromosome

External links

• PDB: RCSB:1a35, PDBe:1a35, PDBj:1a35
• PDBsum: 1a35
• PubMed: 9488644
• UniProt: P11387|TOP1_HUMAN DNA topoisomerase 1 (Gene Name=TOP1)