Structure of PDB 1a0i Chain A

Receptor sequence
>1a0iA (length=332) Species: 10760 (Escherichia phage T7) [Search protein sequence]
VNIKTNPFKAVSFVESAIKKALDNAGYLIAEIKYDGVRGNICVDNTANSY
WLSRVSKTIPALEHLNGFDVRWKRLLNDDRCFYKDGFMLDGELMVKGVDF
NTGSGLLRTKWTDTKNQEFHRKKDKVPFKLHTGHLHIKLYAILPLHIVES
GEDCDVMTLLMQEHVKNMLPLLQEYFPEIEWQAAESYEVYDMVELQQLYE
QKRAEGHEGLIVKDPMCIYKRGKKSGWWKMKPENEADGIIQGLVWGTKGL
ANEGKVIGFEVLLESGRLVNATNISRALMDEFTETVKEATLSQWGFFDAC
TINPYDGWACQISYMEETPDGSLRHPSFVMFR
3D structure
PDB1a0i Crystal structure of an ATP-dependent DNA ligase from bacteriophage T7.
ChainA
Resolution2.6 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) K34 K238 K240
Catalytic site (residue number reindexed from 1) K33 K229 K231
Enzyme Commision number 6.5.1.1: DNA ligase (ATP).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ATP A I33 K34 Y35 R39 R55 E93 Y149 I220 K222 W236 K238 I32 K33 Y34 R38 R54 E92 Y140 I211 K213 W227 K229
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003690 double-stranded DNA binding
GO:0003909 DNA ligase activity
GO:0003910 DNA ligase (ATP) activity
GO:0005524 ATP binding
GO:0016874 ligase activity
GO:0046872 metal ion binding
Biological Process
GO:0006260 DNA replication
GO:0006266 DNA ligation
GO:0006281 DNA repair
GO:0006310 DNA recombination

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Molecular Function
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Biological Process
External links
PDB RCSB:1a0i, PDBe:1a0i, PDBj:1a0i
PDBsum1a0i
PubMed8653795
UniProtP00969|DNLI_BPT7 DNA ligase (Gene Name=1.3)

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