Structure of PDB 6og7 Chain 8

Receptor sequence

>6og78 (length=361) Species: 83333 (Escherichia coli K-12)

INPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQPDVWNEPERA
QALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAEL
DALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYL
RWAESRGFKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVR
KSPFDSGGRRHTSFSSAFVYPEVDDDIDIEINPADLRIDVYRTSGAGGQH
VNRTESAVRITHIPTGIVTQCQNDRSQHKNKDQAMKQMKAKLYELEMQKK
NAEKQAMEDNKSDIGWGSQIRSYVLDDSRIKDLRTGVETRNTQAVLDGSL
DQFIEASLKAG

3D structure

• PDB: 6og7 Extensive ribosome and RF2 rearrangements during translation termination.
• Chain: 8
• Resolution: 3.3 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	8	G135 S136 G137 G138 R200 S209 G210 R213 W319 D330 R332	G132 S133 G134 G135 R197 S206 G207 R210 W316 D327 R329
BS02	rna	8	V46 R60 S61 E64 Q133 R240 D242 R245 G248 A249 G250 G251 Q252 H253 V254 N255 T257 R262 S279 Q280 E311	V43 R57 S58 E61 Q130 R237 D239 R242 G245 A246 G247 G248 Q249 H250 V251 N252 T254 R259 S276 Q277 E308
BS03	rna	8	E172 V173	E169 V170
BS04	rna	8	G137 G138 T139 E140 S205 R212 R213 H214 T215	G134 G135 T136 E137 S202 R209 R210 H211 T212

Gene Ontology

Molecular Function

• GO:0003747 translation release factor activity
• GO:0016149 translation release factor activity, codon specific

Biological Process

• GO:0006412 translation
• GO:0006415 translational termination
• GO:0075523 viral translational frameshifting

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:6og7, PDBe:6og7, PDBj:6og7
• PDBsum: 6og7
• PubMed: 31513010
• UniProt: P07012|RF2_ECOLI Peptide chain release factor RF2 (Gene Name=prfB)