Structure of PDB 8cen Chain 7

Receptor sequence

>8cen7 (length=615) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]

PADFVPDSVSGMFRSHDFSYLRLRPDHASRPLWISPSDGRIILESFSPLA
EQAQDFLVTIAEPISRPSHIHEYKITAYSLYAAVSVGLETDDIISVLDRL
SKVPVAESIINFIKGATISYGKVKLVIKHNRYFVETTQADILQMLLNDSV
IGVHSFEIANESVEVVKKRCQEIDYPVLEEYDFRNDHRNPDLDIDLKPST
QIRPYQEKSLSKMFGNGRARSGIIVLPCGAGKTLVGITAACTIKKSVIVL
CTSSVSVMQWRQQFLQWCTLQPENCAVFTSDNKEMFQTESGLVVSTYSMV
ANTRNRSHDSQKVMDFLTGREWGFIILDEVHVVPAAMFRRVVSTIAAHAK
LGLTATLVREDDKIGDLNFLIGPKLYEANWMELSQKGHIANVQCAEVWCP
MTAEFYQEYLRETARKRMLLYIMNPTKFQACQFLIQYHERRGDKIIVFSD
NVYALQEYALKMGKPFIYGSTPQQERMNILQNFQYNDQINTIFLSKVGDT
SIDLPEATCLIQISSHYGSRRQEAQRLGRILRAKRRNDEGFNAFFYSLVS
KDTQEMYYSTKRQAFLVDQGYAFKVITHLHGMENIPNLAYASPRERRELL
QEVLLKNHPLIRKMY

3D structure

PDB

8cen Yeast PIC-Mediator structure with RNA polymerase II C-terminal domain.

Chain

Resolution

3.0 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

5.6.2.4: DNA 3'-5' helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	7	R464 A495 A496 M497 F498 R519 E520 T573 A574 H676 Y677 S679 Y718 K721	R304 A335 A336 M337 F338 R359 E360 T413 A414 H516 Y517 S519 Y558 K561
BS02	dna	7	S413 S440 K443 T456 S458 M459 R464 S470 N611 V612 G629 S655 K656 V657 H676	S253 S280 K283 T296 S298 M299 R304 S310 N451 V452 G469 S495 K496 V497 H516

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003678	DNA helicase activity
GO:0004386	helicase activity
GO:0005524	ATP binding
GO:0015616	DNA translocase activity
GO:0016787	hydrolase activity
GO:0016853	isomerase activity
GO:0016887	ATP hydrolysis activity
GO:0043138	3'-5' DNA helicase activity

	Biological Process
GO:0000019	regulation of mitotic recombination
GO:0001111	RNA polymerase II promoter clearance
GO:0001113	transcription open complex formation at RNA polymerase II promoter
GO:0001174	transcriptional start site selection at RNA polymerase II promoter
GO:0006281	DNA repair
GO:0006289	nucleotide-excision repair
GO:0006366	transcription by RNA polymerase II
GO:0006367	transcription initiation at RNA polymerase II promoter
GO:0016973	poly(A)+ mRNA export from nucleus
GO:0032508	DNA duplex unwinding

	Cellular Component
GO:0000112	nucleotide-excision repair factor 3 complex
GO:0000439	transcription factor TFIIH core complex
GO:0005634	nucleus
GO:0005675	transcription factor TFIIH holo complex
GO:0005829	cytosol
GO:0097550	transcription preinitiation complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8cen, PDBe:8cen, PDBj:8cen
PDBsum	8cen
PubMed	37014863
UniProt	Q00578\|RAD25_YEAST General transcription and DNA repair factor IIH helicase/translocase subunit XPB/SSL2 (Gene Name=SSL2)

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