Structure of PDB 7d4i Chain 5H

Receptor sequence

>7d4i5H (length=95) Species: 559292 (Saccharomyces cerevisiae S288C)

TDRFKGDDDIPYKERLFERQQGKRAINYQILKNKGLTPKRNKDNRNSRVK
KRKKYQKAQKKLKSVRAVYSGGQSGVYEGEKTGIKKGLTRSVKFK

3D structure

• PDB: 7d4i Cryo-EM structure of 90S small ribosomal precursors complex with Dhr1
• Chain: 5H
• Resolution: 4.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	5H	Q587 S588 G589	Q73 S74 G75
BS02	rna	5H	Y442 Y542 K548 K553 R554 K556 N558 R559 N560 R562 V563 K565 R566 K568 K574 K575 S578 K595 K599	Y12 Y28 K34 K39 R40 K42 N44 R45 N46 R48 V49 K51 R52 K54 K60 K61 S64 K81 K85

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0005515 protein binding
• GO:0042802 identical protein binding

Biological Process

• GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0032040 small-subunit processome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7d4i, PDBe:7d4i, PDBj:7d4i
• PDBsum: 7d4i
• UniProt: Q12136|SAS10_YEAST Something about silencing protein 10 (Gene Name=SAS10)