Structure of PDB 8h6l Chain 5D

Receptor sequence
>8h6l5D (length=1696) Species: 9606 (Homo sapiens) [Search protein sequence]
LPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFK
TLNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDG
TINVDDFKIIYIAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEE
ISATQIIVCTPEKWDIITRKGGERTYTQLVRLIILDEIHLLHDDRGPVLE
ALVARAIRNIEMTQEDVRLIGLSATLPNYEDVATFLRVDPAKGLFYFDNS
FRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQVLVFVHSRK
ETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLP
YGFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIK
GTQVYSPEKGRWTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYY
LSLLNQQLPIESQMVSKLPDMLNAEIVLGNVQNAKDAVNWLGYAYLYIRM
LRSPTLYGISHDDLKGDPLLDQRRLDLVHTAALMLDKNNLVKYDKKTGNF
QVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSLSSEFKNITV
REEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMA
DMVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPL
RQFRKLPEEVVKKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVH
LFPKLELSVHLQPITRSTLKVELTITPDFQWDEKVHGSSEAFWILVEDVD
SEVILHHEYFLLKAKYAQDEHLITFFVPVFEPLPPQYFIRVVSDRWLSCE
TQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESLYQDKFPFFN
PIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYI
TPMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTP
EKWDILSRRWKQRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISS
QIERPIRIVALSSSLSNAKDVAHWLGCSATSTFNFHPNVRPVPLELHIQG
FNISHTQTRLLSMAKPVYHAITKHSPKKPVIVFVPSRKQTRLTAIDILTT
CAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYLHEGLSPMER
RLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDY
PIYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLD
HCMHDHFNAEIVTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHR
HLSDHLSELVEQTLSDLEQSKCISIEDEMDVAPLNLGMIAAYYYINYTTI
ELFSMSLNAKTKVRGLIEIISNAAEYENIPIRHHEDNLLRQLAQKVPHKL
NNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAIRLIQACVDV
LSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGV
ESVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSI
RSGGPVVVLVQLEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISI
KRLTLQQKAKVKLDFVAPATGAHNYTLYFMSDAYMGCDQEYKFSVD
3D structure
PDB8h6l Structural Insights into Human Exon-defined Spliceosome Prior to Activation
Chain5D
Resolution2.6 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.6.4.13: RNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna 5D M544 R545 T570 T589 E591 I595 K599 K696 H726 S727 R728 K729 A787 T812 A813 T814 Y834 T1028 Y1083 R1090 M115 R116 T141 T160 E162 I166 K170 K267 H297 S298 R299 K300 A358 T383 A384 T385 Y405 T599 Y654 R661
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0003724 RNA helicase activity
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0042802 identical protein binding
Biological Process
GO:0000354 cis assembly of pre-catalytic spliceosome
GO:0000388 spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)
GO:0000398 mRNA splicing, via spliceosome
GO:0001649 osteoblast differentiation
GO:0006397 mRNA processing
GO:0008380 RNA splicing
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005681 spliceosomal complex
GO:0005682 U5 snRNP
GO:0016020 membrane
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071005 U2-type precatalytic spliceosome
GO:0071006 U2-type catalytic step 1 spliceosome
GO:0071013 catalytic step 2 spliceosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8h6l, PDBe:8h6l, PDBj:8h6l
PDBsum8h6l
PubMed38658629
UniProtO75643|U520_HUMAN U5 small nuclear ribonucleoprotein 200 kDa helicase (Gene Name=SNRNP200)

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