Structure of PDB 8kg6 Chain 5

Receptor sequence
>8kg65 (length=677) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
NDDDNTEIIKSFKNFILEFRLDSQFIYRDQLRNNILVKNYSLTVNMEHLI
GYNEDIYKKLSDEPSDIIPLFETAITQVAKRISILSRNSLPTFQLILNSN
ANQIPLRDLDSEHVSKIVRLSGIIISTSVLSSRATYLSIMCRNCRHTTSI
TINNFNSIVSLPRSCLSKNCGPDPYIIIHESSKFIDQQFLKLQEIPELVP
VGEMPRNLTMTCDRYLTNKVIPGTRVTIVGIYSIYNSKNSGVAIRTPYIK
ILGIQSDVETSSIWNSVTMFTEEEEEEFLQLSRNPKLYEILTNSIAPSIF
GNEDIKKAIVCLLMGGSKKILPDGMRLRGDINVLLLGDPGTAKSQLLKFV
EKVSPIAVYTSGKGSSAAGLTASVQRDPMTREFYLEGGAMVLADGGVVCI
DEFDKMRDEDRVAIHEAMEQQTISIAKAGITTVLNSRTSVLAAANPIYGR
YDDLKSPGDNIDFQTTILSRFDMIFIVKDDHNEERDISIANHVINIHTGN
ANAMQNQQEENGSEISIEKMKRYITYCRLKCAPRLSPQAAEKLSSNFVTI
RKQLLINELESTERSSIPITIRQLEAIIRITESLAKLELSPIAQERHVDE
AIRLFQASTMDAASQDPSLSEIRRFEQELKRRLPIGWSTSYQTLRREFAL
DKALYALEKHETIQLRHQGQNIYRSGV
3D structure
PDB8kg6 Synergism between CMG helicase and leading strand DNA polymerase at replication fork.
Chain5
Resolution3.07 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.6.4.12: DNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna 5 S445 A447 R455 R460 F462 K506 A507 S366 A368 R376 R381 F383 K427 A428
BS02 ADP 5 R651 E654 R572 E575
BS03 ZN 5 C183 C186 C211 C236 C141 C144 C165 C170
BS04 AGS 5 F379 P418 G419 T420 A421 K422 S423 N524 F300 P339 G340 T341 A342 K343 S344 N445
BS05 MG 5 S423 D480 S344 D401
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003688 DNA replication origin binding
GO:0003697 single-stranded DNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0017116 single-stranded DNA helicase activity
GO:0043138 3'-5' DNA helicase activity
Biological Process
GO:0000727 double-strand break repair via break-induced replication
GO:0006260 DNA replication
GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006270 DNA replication initiation
GO:0006279 premeiotic DNA replication
GO:0006368 transcription elongation by RNA polymerase II
GO:0030174 regulation of DNA-templated DNA replication initiation
GO:0031507 heterochromatin formation
GO:0031509 subtelomeric heterochromatin formation
GO:0032508 DNA duplex unwinding
GO:0033260 nuclear DNA replication
Cellular Component
GO:0000781 chromosome, telomeric region
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005656 nuclear pre-replicative complex
GO:0005737 cytoplasm
GO:0031261 DNA replication preinitiation complex
GO:0031298 replication fork protection complex
GO:0042555 MCM complex
GO:0043596 nuclear replication fork
GO:0071162 CMG complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8kg6, PDBe:8kg6, PDBj:8kg6
PDBsum8kg6
PubMed37730685
UniProtP29496|MCM5_YEAST Minichromosome maintenance protein 5 (Gene Name=MCM5)

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