Structure of PDB 7pfo Chain 5

Receptor sequence

>7pfo5 (length=605) Species: 9606 (Homo sapiens)

ADEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELKRHYNLGEYWI
EVEMEDLASFDEDLADYLYKQPAEHLQLLEEAAKEVADEVTRPRPSGEEV
LQDIQVMLKSDASPSSIRSLKSDMMSHLVKIPGIIIAASAVRAKATRISI
QCRSCRNTLTNIAMRPGLEGYALPRKCCPLDPYFIMPDKCKCVDFQTLKL
QELPDAVPHGEMPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIKKFVGVGI
RSSYIRVLGIQVDTGAVSPQEEEEFRRLAALPNVYEVISKSIAPSIFGGT
DMKKAIACLLFGGSRKRLPDGLTRRGDINLLMLGDPGTAKSQLLKFVEKC
SPIGVYTSGKGSSAAGLTASVMRDPSSRNFIMEGGAMVLADGGVVCIDEF
DKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDE
TKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLHVSALTQT
QAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHE
RDSDRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVS
TLDAA

3D structure

• PDB: 7pfo Structure of a human replisome shows the organisation and interactions of a DNA replication machine.
• Chain: 5
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.12: DNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	5	S410 V418 F427 K471 A472	S363 V371 F380 K424 A425
BS02	ANP	5	R371 E463 R513 V610 R611 E614	R324 E416 R466 V563 R564 E567
BS03	ZN	5	C172 C197	C152 C177
BS04	ANP	5	I343 F344 P383 G384 T385 A386 K387 S388 Q389 L532	I296 F297 P336 G337 T338 A339 K340 S341 Q342 L485
BS05	MG	5	S388 D445 E446	S341 D398 E399

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003688 DNA replication origin binding
• GO:0003697 single-stranded DNA binding
• GO:0004386 helicase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0017116 single-stranded DNA helicase activity
• GO:0043138 3'-5' DNA helicase activity

Biological Process

• GO:0000727 double-strand break repair via break-induced replication
• GO:0006260 DNA replication
• GO:0006268 DNA unwinding involved in DNA replication
• GO:0006270 DNA replication initiation
• GO:0030174 regulation of DNA-templated DNA replication initiation
• GO:0032508 DNA duplex unwinding

Cellular Component

• GO:0000781 chromosome, telomeric region
• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005694 chromosome
• GO:0016020 membrane
• GO:0032991 protein-containing complex
• GO:0042555 MCM complex
• GO:0043231 intracellular membrane-bounded organelle
• GO:0071162 CMG complex

External links

• PDB: RCSB:7pfo, PDBe:7pfo, PDBj:7pfo
• PDBsum: 7pfo
• PubMed: 34694004
• UniProt: P33992|MCM5_HUMAN DNA replication licensing factor MCM5 (Gene Name=MCM5)