Structure of PDB 7ase Chain 5

Receptor sequence

>7ase5 (length=421) Species: 5693 (Trypanosoma cruzi)

QATVNIGTIGHVAHGKSTVVKALSGVKTQKYHREAVMNITIHLGYANAKV
FKCDKCELPAAFHAFPSSQPDKTDCPTCGSPLTLKRHFSFVDCPGHDVLM
ATMLNGAAIMDAALLLIAANEPFPQPQTLEHLKAVEIMRLANLVILQNKI
DLVGEVHAQDQYHKIRNYIDSTIGSNIPIIPISAQLKRNIDYLLEYLCHI
PLPTRQLNCPAHMTVVRSFDINKPGEVDIENLRGGVAGGTVTRGIIRVNQ
VLEIRPGQVHAQTGGTFSCTPLRTRALTLKAEDNSLQYAVPGGLIAVGTT
LDPTLTRQDKMVGHMIADEGSLPEVYAEIEVQYFLFEEMVGRSKQRDRNA
KRVQKLNLQETLQINVGTLTAGATVVNITKNPDIAKLTLVTPVCCTLDEH
IAISRLVEKNFRLIGWGIIRR

3D structure

• PDB: 7ase Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts.
• Chain: 5
• Resolution: 3.33 Å

Enzymatic activity

• Enzyme Commision number: 3.6.5.3: protein-synthesizing GTPase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	5	Q78 K79 Y80 F268 D269 K272 R282 V285 K329 A345 Q357	Q29 K30 Y31 F219 D220 K223 R233 V236 K280 A296 Q308
BS02	MG	5	S66 T89	S17 T40
BS03	GNP	5	V61 G64 K65 S66 T67 N197 K198 L201 Q234	V12 G15 K16 S17 T18 N148 K149 L152 Q185

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003743 translation initiation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding
• GO:0016787 hydrolase activity

Biological Process

• GO:0001731 formation of translation preinitiation complex
• GO:0006412 translation
• GO:0006413 translational initiation

Cellular Component

• GO:0005829 cytosol
• GO:0005850 eukaryotic translation initiation factor 2 complex

External links

• PDB: RCSB:7ase, PDBe:7ase, PDBj:7ase
• PDBsum: 7ase
• PubMed: 33357443
• UniProt: Q4CPV7