Structure of PDB 6rfr Chain 4

Receptor sequence

>6rfr4 (length=486) Species: 4952 (Yarrowia lipolytica)

MFLTSILLSSLYLFNRILAWQGNVKHFYLFASNLLLLFIVVLYINFNTFS
NSFQFNFELFNSLNPFGLSNSDISNGLLFGIDGLSLTFILLTVLLIPLTL
LGNWYNINFNSNLYYTLVLAIGLVILLNFWALDYISFYILFEATLPLLFI
LIHIYGSSDSERASFYVLMFTLSGSLFMLLSIVVISIVLNTTNFINHNLF
VLSLDLQTIIWLGLFIAIMVKTPLFPIHVWLPVVHSESPLAGSMILAGLI
LKLALYAILRLLLPLLCEAQILYTPMIYIISLLTIILTSLATLRQIDLKV
IIAYSSISHMGIAILGVCSNTSLGIYGSIVLGVAHGFVSPALFLIVGGIL
YDRYHIRIVNYYKGLTTYMPQLATYIIILSFANIGTPLTGNFTGEFLSLQ
GGFIRNPIIGGISCISVLLAAIYQLKLTNKLTGGISSIYMHRTNDVTIRE
KFIMNILIISTLIIGICPQIMYNLLYWTVNNYIYII

3D structure

• PDB: 6rfr High-resolution cryo-EM structures of respiratory complex I: Mechanism, assembly, and disease.
• Chain: 4
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.2: NADH:ubiquinone reductase (H(+)-translocating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	LMN	4	L180 V184	L180 V184
BS02	T7X	4	F165 M169 F170	F165 M169 F170

Gene Ontology

Molecular Function

• GO:0003954 NADH dehydrogenase activity
• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0048039 ubiquinone binding

Biological Process

• GO:0009060 aerobic respiration
• GO:0015990 electron transport coupled proton transport
• GO:0042773 ATP synthesis coupled electron transport

Cellular Component

• GO:0005739 mitochondrion
• GO:0016020 membrane
• GO:0031966 mitochondrial membrane
• GO:0045271 respiratory chain complex I

External links

• PDB: RCSB:6rfr, PDBe:6rfr, PDBj:6rfr
• PDBsum: 6rfr
• PubMed: 31844670
• UniProt: Q9B6D6|NU4M_YARLI NADH-ubiquinone oxidoreductase chain 4 (Gene Name=ND4)