Structure of PDB 6gsk Chain 32

Receptor sequence

>6gsk32 (length=208) Species: 300852 (Thermus thermophilus HB8)

GRYIGPVCRLCRREGVKLYLKGERCYSPKCAMERRPYPPGQHGQKRARRP
SDYAVRLREKQKLRRIYGISERQFRNLFEEASKKKGVTGSVFLGLLESRL
DNVVYRLGFAVSRRQARQLVRHGHITVNGRRVDLPSYRVRPGDEIAVAEK
SRNLELIRQNLEAMKGRKVGPWLSLDVEGMKGKFLRLPDREDLALPVNEQ
LVIEFYSR

3D structure

• PDB: 6gsk Tautomeric G•U pairs within the molecular ribosomal grip and fidelity of decoding in bacteria.
• Chain: 32
• Resolution: 3.36 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	32	G2 I5 V8 C9 R10 R13 R14 E24 R25 K30 R36 Y38 P40 G41 Q42 H43 Q45 Y54 L58 R59 Q62 R65 R66 S71 E72 R73 Q74 K84 K86 S113 R115 Q116 R118 Q119 R122 H123 H125 R132 D134 L135 S137 L155 R209	G1 I4 V7 C8 R9 R12 R13 E23 R24 K29 R35 Y37 P39 G40 Q41 H42 Q44 Y53 L57 R58 Q61 R64 R65 S70 E71 R72 Q73 K83 K85 S112 R114 Q115 R117 Q118 R121 H122 H124 R131 D133 L134 S136 L154 R208
BS02	MG	32	S83 K85	S82 K84
BS03	SF4	32	C9 C12 Y20 L21 K22 C26 C31 M33	C8 C11 Y19 L20 K21 C25 C30 M32

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006412 translation
• GO:0042274 ribosomal small subunit biogenesis

Cellular Component

• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6gsk, PDBe:6gsk, PDBj:6gsk
• PDBsum: 6gsk
• PubMed: 29931292
• UniProt: P80373|RS4_THET8 Small ribosomal subunit protein uS4 (Gene Name=rpsD)