Structure of PDB 5e81 Chain 32

Receptor sequence

>5e8132 (length=208) Species: 300852 (Thermus thermophilus HB8)

GRYIGPVCRLCRREGVKLYLKGERCYSPKCAMERRPYPPGQHGQKRARRP
SDYAVRLREKQKLRRIYGISERQFRNLFEEASKKKGVTGSVFLGLLESRL
DNVVYRLGFAVSRRQARQLVRHGHITVNGRRVDLPSYRVRPGDEIAVAEK
SRNLELIRQNLEAMKGRKVGPWLSLDVEGMKGKFLRLPDREDLALPVNEQ
LVIEFYSR

3D structure

• PDB: 5e81 Novel base-pairing interactions at the tRNA wobble position crucial for accurate reading of the genetic code.
• Chain: 32
• Resolution: 2.95 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	32	G2 R3 I5 V8 C9 R10 R13 R14 K22 E24 R25 K30 R36 Y38 P40 G41 Q42 H43 Q45 Y54 A55 L58 R59 Q62 S71 E72 R73 Q74 N77 K84 S113 R115 Q116 R118 Q119 H123 H125 R132 D134 L135 S137 L155 E156 L157 S208 R209	G1 R2 I4 V7 C8 R9 R12 R13 K21 E23 R24 K29 R35 Y37 P39 G40 Q41 H42 Q44 Y53 A54 L57 R58 Q61 S70 E71 R72 Q73 N76 K83 S112 R114 Q115 R117 Q118 H122 H124 R131 D133 L134 S136 L154 E155 L156 S207 R208
BS02	SF4	32	C9 C12 K18 Y20 L21 K22 C26 C31	C8 C11 K17 Y19 L20 K21 C25 C30

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006412 translation
• GO:0042274 ribosomal small subunit biogenesis

Cellular Component

• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5e81, PDBe:5e81, PDBj:5e81
• PDBsum: 5e81
• PubMed: 26791911
• UniProt: P80373|RS4_THET8 Small ribosomal subunit protein uS4 (Gene Name=rpsD)