Structure of PDB 5u8s Chain 3

Receptor sequence
>5u8s3 (length=591) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
PDAVFGDRVRRFQEFLDTFTSYRDSVRSIQVYNSNNAANYNILPHRIIIS
LDDLREFDRSFWSGILVEPAYFIPPAEKALTDLADSMDDVPHHPWKLSFK
GSFGAHALSPRTLTAQHLNKLVSVEGIVTKTSLVRPKLIRSVHYAAKTGR
FHYRDYTDATTTLTTRIPTPAIYPTEDTEGNKLTTEYGYSTFIDHQRITV
QEMPEMAPAGQLPRSIDVILDDDLVDKTKPGDRVNVVGVFKSLGAGGMNQ
SNLIGFKTLILGNTVYPLHAARQMLTDFDIRNINKLSKKKDIFDILSQSL
APSIYGHDHIKKAILLMLMGGVEKNLENGSHLRGDINILMVGDPSTAKSQ
LLRFVLNTASLAIATTGRGSSGVGLTAAVTTDRETGERRLEAGAMVLADR
GVVCIDEFDKMTDVDRVAIHEVMEQQTVTIAKAGIHTTLNARCSVIAAAN
PVFGQYDVNRDPHQNIALPDSLLSRFDLLFVVTDDINEIRDRSISEHVLR
THRVTIPFLRKYVQYAKERVIPQLTQEAINVIVKNYTDLRNDDNTKKSPI
TARTLETLIRLATAHAKVRLSKTVNKVDAKVAANLLRFALL
3D structure
PDB5u8s Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation.
Chain3
Resolution6.1 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.6.4.12: DNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna 3 S438 A444 A445 T447 T448 K499 S371 A377 A378 T380 T381 K432
BS02 ATP 3 S370 I371 Y372 S412 T413 A414 K415 S416 Q417 N517 S303 I304 Y305 S345 T346 A347 K348 S349 Q350 N450
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003688 DNA replication origin binding
GO:0003697 single-stranded DNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0017116 single-stranded DNA helicase activity
GO:1904931 MCM complex binding
Biological Process
GO:0000727 double-strand break repair via break-induced replication
GO:0006260 DNA replication
GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006270 DNA replication initiation
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006279 premeiotic DNA replication
GO:0030466 silent mating-type cassette heterochromatin formation
GO:0031509 subtelomeric heterochromatin formation
GO:0032508 DNA duplex unwinding
GO:1902975 mitotic DNA replication initiation
Cellular Component
GO:0000781 chromosome, telomeric region
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005656 nuclear pre-replicative complex
GO:0005737 cytoplasm
GO:0031261 DNA replication preinitiation complex
GO:0031298 replication fork protection complex
GO:0042555 MCM complex
GO:0043596 nuclear replication fork
GO:0071162 CMG complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5u8s, PDBe:5u8s, PDBj:5u8s
PDBsum5u8s
PubMed28096349
UniProtP24279|MCM3_YEAST DNA replication licensing factor MCM3 (Gene Name=MCM3)

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