Structure of PDB 8ev7 Chain 2g

Receptor sequence

>8ev72g (length=155) Species: 300852 (Thermus thermophilus HB8)

ARRRRAEVRQLQPDLVYGDVLVTAFINKIMRDGKKNLAARIFYDACKIIQ
EKTGQEPLKVFKQAVENVKPRMEVRSRRVGGANYQVPMEVSPRRQQSLAL
RWLVQAANQRPERRAAVRIAHELMDAAEGKGGAVKKKEDVERMAEANRAY
AHYRW

3D structure

• PDB: 8ev7 Molecular basis of the pleiotropic effects by the antibiotic amikacin on the ribosome.
• Chain: 2g
• Resolution: 2.89 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	2g	A2 R3 R4 R6 A7 R10 N28 K29 I30 M31 R32 D33 G34 K35 K36 N37 L38 R41 R79 R94 R95 S98 R102 R114 R115 A116 R119	A1 R2 R3 R5 A6 R9 N27 K28 I29 M30 R31 D32 G33 K34 K35 N36 L37 R40 R78 R93 R94 S97 R101 R113 R114 A115 R118
BS02	rna	2g	Q86 D140 R143 M144 N148	Q85 D139 R142 M143 N147
BS03	MG	2g	R10 L12	R9 L11
BS04	MG	2g	R32 D33	R31 D32

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:0022627 cytosolic small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8ev7, PDBe:8ev7, PDBj:8ev7
• PDBsum: 8ev7
• PubMed: 37537169
• UniProt: P17291|RS7_THET8 Small ribosomal subunit protein uS7 (Gene Name=rpsG)