Structure of PDB 8ife Chain 2M

Receptor sequence

>8ife2M (length=175) Species: 9606 (Homo sapiens)

KASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQL
KKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLT
TAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIK
FPLPHRVLRRQHKPRFTTKRPNTFF

3D structure

• PDB: 8ife Direct visualization of ribosomes in the cell-free system revealed the functional evolution of aminoglycoside.
• Chain: 2M
• Resolution: 2.57 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	2M	S4 G5 R8 Y63 Q66 V67 F68 K70 P72 L73 R87 S88 H91 Y94 R95 R98 R111 G114 A115 R116 H117 R118 R120 R139 H146 D147 S148 P155 H156 R157 V158 R160 R161 H163 R166 T169 R171 P172 T174 F175 F176	S3 G4 R7 Y62 Q65 V66 F67 K69 P71 L72 R86 S87 H90 Y93 R94 R97 R110 G113 A114 R115 H116 R117 R119 R138 H145 D146 S147 P154 H155 R156 V157 R159 R160 H162 R165 T168 R170 P171 T173 F174 F175
BS02	rna	2M	S42 R43 Y46 S49 K53 K55 K56 S57 R120 H122	S41 R42 Y45 S48 K52 K54 K55 S56 R119 H121
BS03	MG	2M	F68 K70	F67 K69

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0016020 membrane
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8ife, PDBe:8ife, PDBj:8ife
• PDBsum: 8ife
• PubMed: 38227611
• UniProt: Q02543|RL18A_HUMAN Large ribosomal subunit protein eL20 (Gene Name=RPL18A)