Structure of PDB 8fc1 Chain 20

Receptor sequence

>8fc120 (length=77) Species: 300852 (Thermus thermophilus HB8)

GSTRNGRDSQAKRLGVKRYEGQVVRAGNILVRQRGTRFKPGKNVGMGRDF
TLFALVDGVVEFQDRGRLGRYVHVRPL

3D structure

• PDB: 8fc1 Structural insights into the mechanism of overcoming Erm-mediated resistance by macrolides acting together with hygromycin-A.
• Chain: 20
• Resolution: 2.5 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	20	S9 T10 N12 R14 D15 S16 Q17 A18 K19 R20 K24 Y26 E27 Q29 R32 A33 G34 N35 I36 R39 R41 G42 T43 G54 R55 D56 F57 F60 F69 R74 L75 R77	S2 T3 N5 R7 D8 S9 Q10 A11 K12 R13 K17 Y19 E20 Q22 R25 A26 G27 N28 I29 R32 R34 G35 T36 G47 R48 D49 F50 F53 F62 R67 L68 R70
BS02	rna	20	R72 R74	R65 R67
BS03	MG	20	G13 R14	G6 R7

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fc1, PDBe:8fc1, PDBj:8fc1
• PDBsum: 8fc1
• PubMed: 37452045
• UniProt: P60493|RL27_THET8 Large ribosomal subunit protein bL27 (Gene Name=rpmA)