Structure of PDB 8w7m Chain 2

Receptor sequence
>8w7m2 (length=627) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
EELTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYG
ARIRTLGEMNSESLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEA
TELHYPDYARIHSEIHVRISDFPTIYSLRELRESNLSSLVRVTGVVTRRT
GVFPQLKYVKFNCLKCGSILGPFFQDSNEEIRISFCTNCKSKGPFRVNGE
KTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKPGEEVEVTG
IYKNNYDGNLNAKNGFPVFATIIEANSIKRRFSWTEEEEREFRKISRDRG
IIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVSIRGDINVLLLGDPG
TAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDEWTLEGGALVLAD
KGVCLIDEFDQDRTSIHEAMEQQSISISKAGIVTTLQARCSIIAAANPNG
GRYNSTLPLAQNVSLTEPILSRFDILCVVRDLVDEEADERLATFVVDSHV
RSHPENLQRQRKKEEEISPIPQELLMKYIHYARTKIYPKLHQMDMDKVSR
VYADLRRESISTPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKVV
VDSFVDAQKVSVRRQLRRSFAIYTLGH
3D structure
PDB8w7m Synergism between CMG helicase and leading strand DNA polymerase at replication fork.
Chain2
Resolution4.12 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.6.4.12: DNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ADP 2 I505 Y506 H508 P545 G546 T547 A548 K549 S550 Q551 L695 I313 Y314 H316 P349 G350 T351 A352 K353 S354 Q355 L491
BS02 ZN 2 C341 C364 C367 C163 C186 C189
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003688 DNA replication origin binding
GO:0003697 single-stranded DNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0017116 single-stranded DNA helicase activity
GO:0043138 3'-5' DNA helicase activity
GO:0046872 metal ion binding
Biological Process
GO:0000727 double-strand break repair via break-induced replication
GO:0006260 DNA replication
GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006270 DNA replication initiation
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006279 premeiotic DNA replication
GO:0006974 DNA damage response
GO:0032508 DNA duplex unwinding
GO:1902975 mitotic DNA replication initiation
GO:1905775 negative regulation of DNA helicase activity
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005656 nuclear pre-replicative complex
GO:0005737 cytoplasm
GO:0031261 DNA replication preinitiation complex
GO:0031298 replication fork protection complex
GO:0042555 MCM complex
GO:0043596 nuclear replication fork
GO:0071162 CMG complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8w7m, PDBe:8w7m, PDBj:8w7m
PDBsum8w7m
PubMed37730685
UniProtP29469|MCM2_YEAST DNA replication licensing factor MCM2 (Gene Name=MCM2)

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