Structure of PDB 8p62 Chain 2

Receptor sequence
>8p622 (length=662) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
EELTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYG
ARIRTLGEMNSESLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEA
TELHYPDYARIHSEIHVRISDFPTIYSLRELRESNLSSLVRVTGVVTRRT
GVFPQLKYVKFNCLKCGSILGPFFQDSNEEIRISFCTNCKSKGPFRVNGE
KTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKPGEEVEVTG
IYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTE
EEEREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVN
GKHSIRGDINVLLLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTA
SVRKDPITKEWTLEGGALVLADKGVCLIDEFDKMNDQDRTSIHEAMEQQS
ISISKAGIVTTLQARCSIIAAANPNGGRYNSTLPLAQNVSLTEPILSRFD
ILCVVRDLVDEEADERLATFVVDSHVRSHPENLNARQRRLQRQRKKEEEI
SPIPQELLMKYIHYARTKIYPKLHQMDMDKVSRVYADLRRESISTGSFPI
TVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKVVVDSFVDAQKVSVRR
QLRRSFAIYTLG
3D structure
PDB8p62 Unwinding of a eukaryotic origin of replication visualized by cryo-EM.
Chain2
Resolution3.9 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.6.4.12: DNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ATP 2 Y506 P545 G546 T547 A548 K549 S550 Q551 V699 Y328 P367 G368 T369 A370 K371 S372 Q373 V521
BS02 ZN 2 C344 C367 C166 C189
BS03 ATP 2 R676 R808 E811 R498 R603 E606
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003688 DNA replication origin binding
GO:0003697 single-stranded DNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0017116 single-stranded DNA helicase activity
GO:0043138 3'-5' DNA helicase activity
GO:0046872 metal ion binding
Biological Process
GO:0000727 double-strand break repair via break-induced replication
GO:0006260 DNA replication
GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006279 premeiotic DNA replication
GO:0006974 DNA damage response
GO:1902975 mitotic DNA replication initiation
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005656 nuclear pre-replicative complex
GO:0005737 cytoplasm
GO:0031261 DNA replication preinitiation complex
GO:0031298 replication fork protection complex
GO:0042555 MCM complex
GO:0043596 nuclear replication fork
GO:0071162 CMG complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8p62, PDBe:8p62, PDBj:8p62
PDBsum8p62
PubMed38760633
UniProtP29469|MCM2_YEAST DNA replication licensing factor MCM2 (Gene Name=MCM2)

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