Structure of PDB 8kme Chain 2

Receptor sequence

>8kme2 (length=250) Species: 9606 (Homo sapiens)

IVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPW
DKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDI
ALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETGQP
SVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGG
PFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQF

3D structure

• PDB: 8kme Structures of thrombin retro-inhibited with SEL2711 and SEL2770 as they relate to factor Xa binding.
• Chain: 2
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 3.4.21.5: thrombin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	2	E23 I24 G25 M26 P28 W29 D116 H119 P120 V121 C122 Y134 K135 R137 N159 K202 P204 N205 R206 W207	E8 I9 G10 M11 P13 W14 D113 H116 P117 V118 C119 Y134 K135 R137 N157 K205 P207 N210 R211 W212
BS02	peptide	2	F34 K36 Q38 L65 R73 T74 Y76 I82	F19 K21 Q24 L60 R68 T69 Y71 I78
BS03	peptide	2	Y60A W60D I174 D189 A190 E192 S214 W215 G216 E217 G219	Y47 W50 I172 D192 A193 E195 S219 W220 G221 E222 G223

Gene Ontology

Molecular Function

• GO:0004252 serine-type endopeptidase activity
• GO:0005509 calcium ion binding

Biological Process

• GO:0006508 proteolysis
• GO:0007596 blood coagulation

External links

• PDB: RCSB:8kme, PDBe:8kme, PDBj:8kme
• PDBsum: 8kme
• PubMed: 10089309
• UniProt: P00734|THRB_HUMAN Prothrombin (Gene Name=F2)