Structure of PDB 8kg9 Chain 2

Receptor sequence
>8kg92 (length=668) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
IDPLREELTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETG
RSVYGARIRTLGEMNSESLEVNYRHLAESKAILALFLAKCPEEMLKIFDL
VAMEATELHYPDYARIHSEIHVRISDFPTIYSLRELRESNLSSLVRVTGV
VTRRTGVFPQLKYVKFNCLKCGSILGPFFQDSNEEIRISFCTNCKSKGPF
RVNGEKTVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKPGEE
VEVTGIYKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDV
FSWTEEEEREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGV
PKNVNGKHSIRGDINVLLLGDPGTAKSQILKYVEKTAHRAVFATGQGASA
VGLTASVRKDPITKEWTLEGGALVLADKGVCLIDEFDKMNDQDRTSIHEA
MEQQSISISKAGIVTTLQARCSIIAAANPNGGRYNSTLPLAQNVSLTEPI
LSRFDILCVVRDLVDEEADERLATFVVDSHVRSHPENLNARQRRLQRQRK
KEEEISPIPQELLMKYIHYARTKIYPKLHQMDMDKVSRVYADLRRESIST
GSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKVVVDSFVDAQK
VSVRRQLRRSFAIYTLGH
3D structure
PDB8kg9 Synergism between CMG helicase and leading strand DNA polymerase at replication fork.
Chain2
Resolution4.52 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.6.4.12: DNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN 2 C344 C364 C367 C171 C191 C194
BS02 ADP 2 I505 Y506 H508 D544 P545 G546 T547 A548 K549 S550 Q551 L695 I332 Y333 H335 D371 P372 G373 T374 A375 K376 S377 Q378 L522
BS03 MG 2 K549 S550 K376 S377
BS04 AGS 2 V807 R808 E811 V607 R608 E611
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003688 DNA replication origin binding
GO:0003697 single-stranded DNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0017116 single-stranded DNA helicase activity
GO:0043138 3'-5' DNA helicase activity
GO:0046872 metal ion binding
Biological Process
GO:0000727 double-strand break repair via break-induced replication
GO:0006260 DNA replication
GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006270 DNA replication initiation
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006279 premeiotic DNA replication
GO:0006974 DNA damage response
GO:0032508 DNA duplex unwinding
GO:1902975 mitotic DNA replication initiation
GO:1905775 negative regulation of DNA helicase activity
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005656 nuclear pre-replicative complex
GO:0005737 cytoplasm
GO:0031261 DNA replication preinitiation complex
GO:0031298 replication fork protection complex
GO:0042555 MCM complex
GO:0043596 nuclear replication fork
GO:0071162 CMG complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8kg9, PDBe:8kg9, PDBj:8kg9
PDBsum8kg9
PubMed37730685
UniProtP29469|MCM2_YEAST DNA replication licensing factor MCM2 (Gene Name=MCM2)

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