Structure of PDB 8kg6 Chain 2

Receptor sequence
>8kg62 (length=662) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
ELTLESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGA
RIRTLGEMNSESLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEAT
ELHYPDYARIHSEIHVRISDFPTIYSLRELRESNLSSLVRVTGVVTRRTG
VFPQLKYVKFNCLKCGSILGPFFQDSNEEIRISFCTNCKSKGPFRVNGEK
TVYRNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKPGEEVEVTGI
YKNNYDGNLNAKNGFPVFATIIEANSIKRREGNTANEGEEGLDVFSWTEE
EEREFRKISRDRGIIDKIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNG
KHSIRGDINVLLLGDPGTAKSQILKYVEKTAHRAVFATGQGASAVGLTAS
VRKDPITKEWTLEGGALVLADKGVCLIDEFDKMNDQDRTSIHEAMEQQSI
SISKAGIVTTLQARCSIIAAANPNGGRYNSTLPLAQNVSLTEPILSRFDI
LCVVRDLVDEEADERLATFVVDSHVRSHPENLNARQRRLQRQRKKEEEIS
PIPQELLMKYIHYARTKIYPKLHQMDMDKVSRVYADLRRESISTGSFPIT
VRHLESILRIAESFAKMRLSEFVSSYDLDRAIKVVVDSFVDAQKVSVRRQ
LRRSFAIYTLGH
3D structure
PDB8kg6 Synergism between CMG helicase and leading strand DNA polymerase at replication fork.
Chain2
Resolution3.07 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.6.4.12: DNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna 2 S572 V574 V580 W589 K633 A634 S393 V395 V401 W410 K454 A455
BS02 ZN 2 C341 C364 C162 C185
BS03 ADP 2 I505 Y506 P545 G546 T547 A548 K549 S550 Q551 L695 V699 I326 Y327 P366 G367 T368 A369 K370 S371 Q372 L516 V520
BS04 AGS 2 H531 Q626 R676 V807 R808 E811 H352 Q447 R497 V601 R602 E605
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003688 DNA replication origin binding
GO:0003697 single-stranded DNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0017116 single-stranded DNA helicase activity
GO:0043138 3'-5' DNA helicase activity
GO:0046872 metal ion binding
Biological Process
GO:0000727 double-strand break repair via break-induced replication
GO:0006260 DNA replication
GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006270 DNA replication initiation
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006279 premeiotic DNA replication
GO:0006974 DNA damage response
GO:0032508 DNA duplex unwinding
GO:1902975 mitotic DNA replication initiation
GO:1905775 negative regulation of DNA helicase activity
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005656 nuclear pre-replicative complex
GO:0005737 cytoplasm
GO:0031261 DNA replication preinitiation complex
GO:0031298 replication fork protection complex
GO:0042555 MCM complex
GO:0043596 nuclear replication fork
GO:0071162 CMG complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8kg6, PDBe:8kg6, PDBj:8kg6
PDBsum8kg6
PubMed37730685
UniProtP29469|MCM2_YEAST DNA replication licensing factor MCM2 (Gene Name=MCM2)

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