Structure of PDB 8b9d Chain 2

Receptor sequence
>8b9d2 (length=576) Species: 9606 (Homo sapiens) [Search protein sequence]
NLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKERISDM
CKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYP
KYDRITNHIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQL
SIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGI
YHNNYDGSLNTANGFPVFATVILANHVAKKGELTDEDVKMITSLSKDQQI
GEKIFASIAPSIYGHEDIKRGLALALFGGEPKNPGGKHKVRGDINVLLCG
DPGTAKSQFLKYIEKVSSRAIFTTGQGASAVGLTAYVQRHPVSREWTLEA
GALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQAR
CTVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDILCVVRDTVDPVQDE
MLARFVVGSHVRHHPSGVEPLPQEVLKKYIIYAKERVHPKLNQMDQDKVA
KMYSDLRKESMATGSIPITVRHIESMIRMAEAHARIHLRDYVIEDDVNMA
IRVMLESFIDTQKFSVMRSMRKTFAR
3D structure
PDB8b9d How Pol alpha-primase is targeted to replisomes to prime eukaryotic DNA replication.
Chain2
Resolution3.4 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.6.4.12: DNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna 2 S552 V560 K613 A614 S329 V337 K390 A391
BS02 ANP 2 P525 G526 T527 A528 K529 S530 Q531 P302 G303 T304 A305 K306 S307 Q308
BS03 ANP 2 R656 V764 R765 E768 R433 V520 R521 E524
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003688 DNA replication origin binding
GO:0003697 single-stranded DNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0017116 single-stranded DNA helicase activity
GO:0019899 enzyme binding
GO:0042393 histone binding
GO:0043138 3'-5' DNA helicase activity
GO:0046872 metal ion binding
Biological Process
GO:0000727 double-strand break repair via break-induced replication
GO:0006139 nucleobase-containing compound metabolic process
GO:0006260 DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006270 DNA replication initiation
GO:0006334 nucleosome assembly
GO:0006915 apoptotic process
GO:0030174 regulation of DNA-templated DNA replication initiation
GO:0032508 DNA duplex unwinding
GO:0071353 cellular response to interleukin-4
GO:0090102 cochlea development
GO:1902975 mitotic DNA replication initiation
GO:1905775 negative regulation of DNA helicase activity
Cellular Component
GO:0000781 chromosome, telomeric region
GO:0000785 chromatin
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005664 nuclear origin of replication recognition complex
GO:0005694 chromosome
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0042555 MCM complex
GO:0071162 CMG complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8b9d, PDBe:8b9d, PDBj:8b9d
PDBsum8b9d
PubMed37506699
UniProtP49736|MCM2_HUMAN DNA replication licensing factor MCM2 (Gene Name=MCM2)

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