Structure of PDB 8fc3 Chain 1e

Receptor sequence

>8fc31e (length=148) Species: 300852 (Thermus thermophilus HB8)

DFEEKMILIRRTARMQAGGRRFRFGALVVVGDRQGRVGLGFGKAPEVPLA
VQKAGYYARRNMVEVPLQNGTIPHEIEVEFGASKIVLKPAAPGTGVIAGA
VPRAILELAGVTDILTKELGSRNPINIAYATMEALRQLRTKADVERLR

3D structure

• PDB: 8fc3 Structural insights into the mechanism of overcoming Erm-mediated resistance by macrolides acting together with hygromycin-A.
• Chain: 1e
• Resolution: 2.6 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	1e	R14 T16 A17 R18 M19 Q20 A21 G22 R24 R25 K47 K57 Y60 F84 G85 A86 A95 T98 A102 G103 L119 T120 K121 E122 L123 S125 R126 N127 N130 Y133	R10 T12 A13 R14 M15 Q16 A17 G18 R20 R21 K43 K53 Y56 F80 G81 A82 A91 T94 A98 G99 L115 T116 K117 E118 L119 S121 R122 N123 N126 Y129
BS02	MG	1e	A86 S87 S125 N130	A82 S83 S121 N126

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0015935 small ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fc3, PDBe:8fc3, PDBj:8fc3
• PDBsum: 8fc3
• PubMed: 37452045
• UniProt: Q5SHQ5|RS5_THET8 Small ribosomal subunit protein uS5 (Gene Name=rpsE)