Structure of PDB 8fc4 Chain 1Q

Receptor sequence

>8fc41Q (length=141) Species: 300852 (Thermus thermophilus HB8)

MLMPRRMKYRKQQRGRLKGATKGGDYVAFGDYGLVALEPAWITAQQIEAA
RVAMVRHFRRGGKIFIRIFPDKPYTKKPLEVRMGKGKGNVEGYVAVVKPG
RVMFEVAGVTEEQAMEALRIAGHKLPIKTKIVRRDAYDEAQ

3D structure

• PDB: 8fc4 Structural insights into the mechanism of overcoming Erm-mediated resistance by macrolides acting together with hygromycin-A.
• Chain: 1Q
• Resolution: 2.45 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	1Q	P4 R5 R6 K8 Y9 K11 Q13 R14 G15 R16 L17 K18 K22 G24 F29 W41 Q46 A49 R56 R59 R60 R67 F69 D71 Y74 K76 K77 E80 R82 M83 G84 K85 G86 K87 R101 H123 K124 L125 P126 K128	P4 R5 R6 K8 Y9 K11 Q13 R14 G15 R16 L17 K18 K22 G24 F29 W41 Q46 A49 R56 R59 R60 R67 F69 D71 Y74 K76 K77 E80 R82 M83 G84 K85 G86 K87 R101 H123 K124 L125 P126 K128
BS02	rna	1Q	R16 L17 G19	R16 L17 G19
BS03	MG	1Q	T75 G88	T75 G88
BS04	MG	1Q	G84 G86	G84 G86
BS05	MG	1Q	R14 Y74 T75	R14 Y74 T75
BS06	MG	1Q	L125 I127	L125 I127
BS07	MG	1Q	T21 V97 K98 R101	T21 V97 K98 R101

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8fc4, PDBe:8fc4, PDBj:8fc4
• PDBsum: 8fc4
• PubMed: 37452045
• UniProt: P60489|RL16_THET8 Large ribosomal subunit protein uL16 (Gene Name=rplP)