Structure of PDB 8ev7 Chain 1G

Receptor sequence

>8ev71G (length=181) Species: 300852 (Thermus thermophilus HB8)

PLDVALKRKYYEEVRPELIRRFGYQNVWEVPRLEKVVINQGLGEAKEDAR
ILEKAAQELALITGQKPAVTRAKKSISNFKLRKGMPIGLRVTLRRDRMWI
FLEKLLNVALPRIRDFRGLNPNSFDGRGNYNLGLREQLIFPEITYDMVDA
LRGMDIAVVTTAETDEEARALLELLGFPFRK

3D structure

• PDB: 8ev7 Molecular basis of the pleiotropic effects by the antibiotic amikacin on the ribosome.
• Chain: 1G
• Resolution: 2.89 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	1G	N40 G42 L43 K47 T71 A73 K74 I77 N79 F80 L82 I88 R91 S124 D126 R128 N130 N132 G134 L135 G154 M155 D156	N39 G41 L42 K46 T70 A72 K73 I76 N78 F79 L81 I87 R90 S123 D125 R127 N129 N131 G133 L134 G153 M154 D155
BS02	rna	1G	P2 N27 V28 W29 Q66 K67 A69 V70 R72 R91 V92 T93 R95 R96 R98	P1 N26 V27 W28 Q65 K66 A68 V69 R71 R90 V91 T92 R94 R95 R97
BS03	rna	1G	S76 I77 S78	S75 I76 S77
BS04	MG	1G	R128 N130 T161 A163	R127 N129 T160 A162
BS05	MG	1G	N123 F125	N122 F124

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8ev7, PDBe:8ev7, PDBj:8ev7
• PDBsum: 8ev7
• PubMed: 37537169
• UniProt: Q5SHQ0|RL5_THET8 Large ribosomal subunit protein uL5 (Gene Name=rplE)