Structure of PDB 6woo Chain 1

Receptor sequence

>6woo1 (length=600) Species: 4932 (Saccharomyces cerevisiae)

KDLRSPICCILGHVDTGKTKLLDKIRQTNVQGGEAGGITQQIGATYFPID
AIKAKTKVMAEYEKQTFDVPGLLVIDTPGHESFSNLRSRGSSLCNIAILV
IDIMHGLEQQTIESIKLLRDRKAPFVVALNKIDRLYDWKAIPNNSFRDSF
AKQSRAVQEEFQSRYSKIQLELAEQGLNSELYFQNKNMSKYVSIVPTSAV
TGEGVPDLLWLLLELTQKRMSKQLMYLSHVEATILEVKVVEGFGTTIDVI
LSNGYLREGDRIVLCGMNGPIVTNIRALLTPQPLRELRLKSEYVHHKEVK
AALGVKIAANDLEKAVSGSRLLVVGPEDDEDELMDDVMDDLTGLLDSVDT
TGKGVVVQASTLGSLEALLDFLKDMKIPVMSIGLGPVYKRDVMKASTMLE
KAPEYAVMLCFDVKVDKEAEQYAEQEGIKIFNADVIYHLFDSFTAYQEKL
LEERRKDFLDYAIFPCVLQTLQIINKRGPMIIGVDVLEGTLRVGTPICAV
KTDPTTKERQTLILGKVISLEINHQPVQEVKKGQTAAGVAVRLEDPSGQQ
PIWGRHVDENDTLYSLVSRRSIDTLKDKAFRDQVARSDWLLLKKLKVVFG

3D structure

• PDB: 6woo An active role of the eukaryotic large ribosomal subunit in translation initiation fidelity.
• Chain: 1
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 3.6.5.3: protein-synthesizing GTPase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	1	H413 E481 H505 Q509 R534 Y837 T935 A936 A937 R942	H13 E81 H105 Q109 R134 Y437 T535 A536 A537 R542
BS02	rna	1	G642 F643 G644 R676 E686 R688 L689 K690 H695 N710 Y788 K789 R790	G242 F243 G244 R276 E286 R288 L289 K290 H295 N310 Y388 K389 R390
BS03	rna	1	I874 N875 K876 R877 P879 S919 E921 N923 A940 R942 R955 H956	I474 N475 K476 R477 P479 S519 E521 N523 A540 R542 R555 H556
BS04	GDP	1	V414 D415 K418 T419 K420 N530 K531 D533 R534 A599 V600	V14 D15 K18 T19 K20 N130 K131 D133 R134 A199 V200
BS05	MET	1	G883 N923 G938	G483 N523 G538

Gene Ontology

Molecular Function

• GO:0003924 GTPase activity
• GO:0005525 GTP binding

External links

• PDB: RCSB:6woo, PDBe:6woo, PDBj:6woo
• PDBsum: 6woo
• UniProt: P39730|IF2P_YEAST Eukaryotic translation initiation factor 5B (Gene Name=FUN12)