Structure of PDB 1iw7 Chain P Binding Site BS08

Receptor Information

>1iw7 Chain P (length=345) Species: 274 (Thermus thermophilus)

KISTSDPVRQYLHEIGQVPLLTLEEEVELARKVEEGMEAIKKLSEITGLD
PDLIREVVRAKILGSARVRHIPGLKETLDPKTVEEIDQKLKSLPKEHKRY
LHIAREGEAARQHLIEANLRLVVSIAKKYTGRGLSFLDLIQEGNQGLIRA
VEKFEYKRRFKFSTYATWWIRQAINRAIADQARTIRIPVHMVETINKLSR
TARQLQQELGREPTYEEIAEAMGPGWDAKRVEETLKIAQEPVSLETPIGD
EKDSFYGDFIPDEHLPSPVDAATQSLLSEELEKALSKLSEREAMVLKLRK
GLIDGEEVGAFFGVTRERIRQIENKALRKLKYHESRTRKLRDFLD

Ligand information

• Ligand ID: MG
• InChI: InChI=1S/Mg/q+2
• InChIKey: JLVVSXFLKOJNIY-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mg+2]
  CACTVS 3.341: [Mg++]
• Formula: Mg
• Name: MAGNESIUM ION
• DrugBank: DB01378
• PDB chain: 1iw7 Chain P Residue 9401

Receptor-Ligand Complex Structure

Structure summary

• PDB: 1iw7 Crystal structure of a bacterial RNA polymerase holoenzyme at 2.6 A resolution
• Resolution: 2.6 Å
• Binding residue (original residue number in PDB): M367 V368 L371 R372
• Binding residue (residue number reindexed from 1): M294 V295 L298 R299
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003700 DNA-binding transcription factor activity
• GO:0016987 sigma factor activity

Biological Process

• GO:0006352 DNA-templated transcription initiation
• GO:0006355 regulation of DNA-templated transcription
• GO:0010468 regulation of gene expression
• GO:2000142 regulation of DNA-templated transcription initiation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1iw7, PDBe:1iw7, PDBj:1iw7
• PDBsum: 1iw7
• PubMed: 12000971
• UniProt: Q72L95|SIGA_THET2 RNA polymerase sigma factor SigA (Gene Name=sigA)