Structure of PDB 8fxt Chain A Binding Site BS08

Receptor Information

>8fxt Chain A (length=305) Species: 562 (Escherichia coli)

ATRIGVTIYKYDDNFMSVVRKAIEQDAKAAPDVQLLMNDSQNDQSKQNDQ
IDVLLAKGVKALAINLVDPAAAGTVIEKARGQNVPVVFFNKEPSRKALDS
YDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNKDGQIQFVLLKGEPG
HPDAEARTTYVIKELNDKGIKTEQLQLDTAMCDTAQAKDKMDAWLSGPNA
NKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALA
GTVLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKD
NLAEF

Ligand information

• Ligand ID: CA
• InChI: InChI=1S/Ca/q+2
• InChIKey: BHPQYMZQTOCNFJ-UHFFFAOYSA-N
• SMILES:
  CACTVS 3.341: [Ca++]
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Ca+2]
• Formula: Ca
• Name: CALCIUM ION
• DrugBank: DB14577
• PDB chain: 8fxt Chain A Residue 408

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8fxt Chromophore carbonyl twisting in fluorescent biosensors encodes direct readout of protein conformations with multicolor switching.
• Resolution: 1.53 Å
• Binding residue (original residue number in PDB): D134 N136 D138 Q140 Q142 E205
• Binding residue (residue number reindexed from 1): D133 N135 D137 Q139 Q141 E204
• Annotation score: 4

Gene Ontology

Molecular Function

• GO:0005509 calcium ion binding
• GO:0030246 carbohydrate binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006935 chemotaxis
• GO:0015757 galactose transmembrane transport
• GO:0015765 methylgalactoside transport

Cellular Component

• GO:0016020 membrane
• GO:0030288 outer membrane-bounded periplasmic space
• GO:0042597 periplasmic space
• GO:0055052 ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing

External links

• PDB: RCSB:8fxt, PDBe:8fxt, PDBj:8fxt
• PDBsum: 8fxt
• PubMed: 37598249
• UniProt: P0AEE5|MGLB_ECOLI D-galactose/methyl-galactoside binding periplasmic protein MglB (Gene Name=mglB)