Structure of PDB 7m4i Chain A Binding Site BS07

Receptor Information

>7m4i Chain A (length=318) Species: 9606 (Homo sapiens)

HNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEACS
IPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQM
WYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQK
AAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSL
RQEGFLTDDLVKGETKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLYF
TGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNHGAKVGPGRVLPTPTEK
DVFRLLGLPYREPAERDW

Ligand information

• Ligand ID: PPV
• InChI: InChI=1S/H4O7P2/c1-8(2,3)7-9(4,5)6/h(H2,1,2,3)(H2,4,5,6)
• InChIKey: XPPKVPWEQAFLFU-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04: O=P(O)(O)OP(=O)(O)O
  CACTVS 3.341: O[P](O)(=O)O[P](O)(O)=O
  OpenEye OEToolkits 1.5.0: OP(=O)(O)OP(=O)(O)O
• Formula: H4 O7 P2
• Name: PYROPHOSPHATE
• ChEMBL: CHEMBL1160571
• DrugBank: DB04160
• ZINC: ZINC000006827695
• PDB chain: 7m4i Chain A Residue 607

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7m4i Watching right and wrong nucleotide insertion captures hidden polymerase fidelity checkpoints.
• Resolution: 2.005 Å
• Binding residue (original residue number in PDB): S417 R420 G426 D427 D429
• Binding residue (residue number reindexed from 1): S166 R169 G175 D176 D178
• Annotation score: 5

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:7m4i, PDBe:7m4i, PDBj:7m4i
• PDBsum: 7m4i
• PubMed: 35680862
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)