Structure of PDB 8r6y Chain A Binding Site BS06

Receptor Information

>8r6y Chain A (length=1991) Species: 992210 (SFTS virus AH12)

MNLEVLCGRINVENGLSLGEPGLYDQIYDRPGLPDLDVTVDATGVTVDIG
AVPDSASQLGSSINAGLITIQLSEAYKINHDFTFSGLSKTTDRRLSEVFP
ITHDGSDGMTPAVIHTRLDGTIVVVEFSTTRSHNIGGLEAAYRTKIEKYR
DPISRRVDIMENPRVFFGVIVVSSGGVLSNMPLTQDEAEELMYRFCIANE
IYTKAREEELEAISRALSFFSLFEPNIERVEGTFPNSEIKMLEQFLSTPA
DVDFITKTLKAKEVEAYADLCDSHYLKPEKTIQERLEINRCEAIDKTQDL
LAGLHARSNKQTSLNRGTVKLPPWLPKPSSESIDIKTDSGFGSLMDHGAY
GELWAKCLLDVSLGNVEGVVSDPAKELDIAISITYRRFKPALSSSARQEF
SLQGVEGKKWKRMAANQKKEKESHETLSPFLDVEDIGDFLTFNNLLTDSR
YGDESIQRAVSILLEKASAMQDTELTHALNDSFKRNLSSNVVQWSLWVSC
LAQELASALKQHCRAGEFIIKKLKFWPIYVIIKPTKSSSHIFYSLGIRKA
DVTRRLTGRVFSDTIDAGEWELTEFKSLKTCKLTNLVNLPCTMLNSIAFW
REKLGVAPWLVRKPCSELREQVGLTFLISLEDKSKTEEIITLTRYTQMEG
FVSPPMLPKPQKMLGKLDGPLRTKLQVYLLRKHLDCMVRIASQPFSLIPR
EGRVEWGGTFHAISGRSTNLENMVNSWYIGYYKNKEESTELNALGEMYKK
IVEMEEDKPSSPEFLGWGDTDSPKKHEFSRSFLRAACSSLEREIAQRHGR
QWKQNLEERVLREIGTKNILDLASMKATSNFSKDWELYSEVQTKEYHRSK
LLEKMATLIEKGVMWYIDAVGQAWKAVLDDGCMRICLFKKNQHGGLREIY
VMDANARLVQFGVETMARCVCELSPHETVANPRLKNSIIENHGLKSARSL
GPGSININSSNDAKKWNQGHYTTKLALVLCWFMPAKFHRFIWAAISMFRR
KKMMVDLRFLAHLSSKSESRSSDPFREAMTDAFHGNRDVSWMDKGRTYIK
TETGMMQGILHFTSSLLHSCVQSFYKSYFVSKLKEGYMGESISGVVDVIE
GSDDSAIMISIRPKSDMDEVRSRFFVANLLHSVKFLNPLFGIYSSEKSTV
NTVYCVEYNSEFHFHRHLVRPTLRWIAASHQISETEALASRQEDYSNLLT
QCLEGGASFSLTYLIQCAQLLHHYMLLGLCLHPLFGTFMGMLISDPDPAL
GFFLMDNPAFAGGAGFRFNLWRACKTTDLGRKYAYYFNEIQGKTKGDEDY
RALDATSGGTLSHSVMVYWGDRKKYQALLNRMGLPEDWVEQIDENPGVLY
RRAANKKELLLKLAEKVHSPGVTSSLSKGHVVPRVVAAGVYLLSRHCFRF
STQKASLIKLLMMSSISAMKHGGSLNPNQERMLFPQAQEYDRVCTLLEEV
EHLTGKFVVRERNIVRSRIDLFQEPVDLRCKAEDLVSEVWFGLKRTKLGP
RLLKEEWDKLRASFAWLSTDPSETLRDGPFLSHVQFRNFIAHVDAKSRSV
RLLGAPVTTISQVVRMNFFPGFSLEAEKSLDNQERLESISILKHVLFMVL
NGPYTEEYKLEMIIEAFSTLVIPQPSEVIRKSRTMTLCLLSNYLSSRGGS
ILDQIERAQSGTLGGFSKPQKTFVRPGGGVGYKGKGVWTGVMEDTHVQIL
IDGDGTSNWLEEIRLSSDARLYDVIESIRRLCDDLGINNRVASAYRGHCM
VRLSGFKIKPASRTDGCPVRIMNPDEVKMRVRGDILNLSVTIQEGRVMNI
LSYRPRDTDISESAAAYLWSNRDLFSFGKKEPSCSWICLKTLDNWAWSHA
SVLLANDRKTQGIDNRAMGNIFRDCLEGSLRKQGLMRSKLTQELVDILEG
SLDIESIFDGAPILWSAEVEEFGEGVVAVSYSSKYYHLTLMDQAAITMCA
IMGKEGCRGLLTEKRCMAAIREQVRPFLIFLQIPEDSISWV

Ligand information

• Ligand ID: MG
• InChI: InChI=1S/Mg/q+2
• InChIKey: JLVVSXFLKOJNIY-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mg+2]
  CACTVS 3.341: [Mg++]
• Formula: Mg
• Name: MAGNESIUM ION
• DrugBank: DB01378
• PDB chain: 8r6y Chain A Residue 2102

Receptor-Ligand Complex Structure

Structure summary

• PDB: 8r6y Structural snapshots of phenuivirus cap-snatching and transcription
• Resolution: 3.4 Å
• Binding residue (original residue number in PDB): D985 A986 D1126
• Binding residue (residue number reindexed from 1): D962 A963 D1103
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.

Gene Ontology

Molecular Function

• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016740 transferase activity
• GO:0016787 hydrolase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0044165 host cell endoplasmic reticulum
• GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
• GO:0044177 host cell Golgi apparatus
• GO:0044423 virion component

External links

• PDB: RCSB:8r6y, PDBe:8r6y, PDBj:8r6y
• PDBsum: 8r6y
• PubMed: 38709882
• UniProt: I0DF35|L_SFTS RNA-directed RNA polymerase L