Structure of PDB 6bk8 Chain A Binding Site BS05

Receptor Information
>6bk8 Chain A (length=1960) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
NLYTPKAEMPPEHLRKIINTHSDMASKMYNTDKKAFLGALKYLPHAILKL
LENMPHPWEQAKEVKVLYHTSGAITFVNETPRVIEPVYTAQWSATWIAMR
REKRDRTHFKRMRFPPFDDDEPPLSYEQHIENIEPLDPINLPLDSQDDEY
VKDWLYDSRPLEEDSKKVNGTSYKKWSFDLPEMSNLYRLSTPLRDEVTDK
NYYYLFDKKSFFNGKALNNAIPGGPKFEPLYPRENEFNSIDRVIFRVPIR
SEYKVAFPHLYNSRPRSVRIPWYNNPVSCIIQNDEEYDTPALFFDPSLNP
IPHGDFTLPEDFAPLLAEEEELILPNTKDAMSLYHSPFPFNRTKGKMVRA
QDVALAKKWFLQHPDEEYPVKVKVSYQKLLKNYVLNELHPTLPTNHNKTK
LLKSLKNTKYFQQTTIDWVEAGLQLCRQGHNMLNLLIHRKGLTYLHLDYN
FNLKPTKTLTTKERKKSRLGNSFHLMRELLKMMKLIVDTHVQFRLGNVDA
FQLADGIHYILNHIGQLTGIYRYKYKVMHQIRACKDLKHIIYYKFNKNLG
KGPGCGFWQPAWRVWLNFLRGTIPLLERYIGNLITRQFEGRSNEIVKTTT
KQRLDAYYDLELRNSVMDDILEMMPESIRQKKARTILQHLSEAWRCWKAN
IPWDVPGMPAPIKKIIERYIKSKADAWVSAAHYNRERIKRGAHVEKTMVK
KNLGRLTRLWIKNEQERQRQIQKNGPEITPEEATTIFSVMVEWLESRSFS
PIPFPPLTYKNDTKILVLALEDLKDVYASKVRLNASEREELALIEEAYDN
PHDTLNRIKKYLLTQRVFKPVDITMMENYQNISPVYSVDPLEKITDAYLD
QYLWYEADQRKLFPNWIKPSDSEIPPLLVYKWTQGINNLSEIWDVSRGQS
AVLLETTLGEMAEKIDFTLLNRLLRLIVDPNIADYITAKNNVVINFKDMS
HVNKYGLIRGLKFASFIFQYYGLVIDLLLLGQERATDLAGPANNPNEFMQ
FKSKEVEKAHPIRLYTRYLDRIYMLFHFEEDEGEELTDEYLAENPDPNFE
NSIGYNNRKCWPKDSRMRLIRQDVNLGRAVFWEIQSRVPTSLTSIKWENA
FVSVYSKNNPNLLFSMCGFEVRILPRQRMEEVVSNDEGVWDLVDERTKQR
TAKAYLKVSEEEIKKFDSRIRGILMASGSTTFTKVAAKWNTSLISLFTYF
REAIVATEPLLDILVKGETRIQNRVKLGLNSKMPTRFPPAVFYTPKELGG
LGMISASHILIPASDLSWSKQTDTGITHFRAGMTHEDEKLIPTIFRYITT
WENEFLDSQRVWAEYATKRQEAIQQNRRLAFEELEGSWDRGIPRISTLFQ
RDRHTLAYDRGHRIRREFKQYSLERNSPFWWTNSHHDGKLWNLNAYRTDV
IQALGGIETILEHTLFKGTGFNSWEGLFWEKASGFEDSMQFKKLTHAQRT
GLSQIPNRRFTLWWSPTINRANVYVGFLVQLDLTGIFLHGKIPTLKISLI
QIFRAHLWQKIHESIVFDICQILDGELDVLQIESVTKETVHPRKSYKMNS
SAADITMESVHEWEVSKPSLLHETNDSFKGLITNKMWFDVQLRYGDYDSH
DISRYVRAKFLDYTTDNVSMYPSPTGVMIGIDLAYNMYDAYGNWFNGLKP
LIQNSMRTIMKANPALYVLRERIRKGLQIYQSSVQEPFLNSSNYAELFNN
DIKLFVDDTNVYRVTVHKTFEGNVATKAINGCIFTLNPKTGHLFLKIIHT
SVWAGQKRLSQLAKWKTAEEVSALVRSLPKEEQPKQIIVTRKAMLDPLEV
HMLDFPNIAIRPTELRLPFSAAMSIDKLSDVVMKATEPQMVLFNIYDDWL
DRISSYTAFSRLTLLLRALKTNEESAKMILLSDPTITIKSYHLWPSFTDE
QWITIESQMRDLILTEYGRKYNVNISALTQTEIKDIILGQNIKAPSVKRQ
KMAELEAARS
Ligand information
>6bk8 Chain i (length=59) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
guauguauuuauuuuagaacuaguuacuaacauuuuuuuuuuaaaaaaua
auuauauag
...................................<.<<<<.>>>>.>..
.........
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB6bk8 Structure of the yeast spliceosomal postcatalytic P complex.
Resolution3.3 Å
Binding residue
(original residue number in PDB)		T607 K611 G1580 F1581 A1593 Q1594 G1597 I1601 R1604 Q1647 Y1826 F1834 Y1858 N1869 V1870 T1872 K1903 R1904 Q1907 R1937 E1960 Y2067 P2091 R2095
Binding residue
(residue number reindexed from 1)		T461 K465 G1434 F1435 A1447 Q1448 G1451 I1455 R1458 Q1501 Y1680 F1688 Y1712 N1723 V1724 T1726 K1757 R1758 Q1761 R1791 E1814 Y1921 P1945 R1949
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0000386 second spliceosomal transesterification activity
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0017070 U6 snRNA binding
GO:0030619 U1 snRNA binding
GO:0030620 U2 snRNA binding
GO:0030623 U5 snRNA binding
GO:0097157 pre-mRNA intronic binding
Biological Process
GO:0000244 spliceosomal tri-snRNP complex assembly
GO:0000350 generation of catalytic spliceosome for second transesterification step
GO:0000387 spliceosomal snRNP assembly
GO:0000389 mRNA 3'-splice site recognition
GO:0000395 mRNA 5'-splice site recognition
GO:0000398 mRNA splicing, via spliceosome
GO:0006397 mRNA processing
GO:0008380 RNA splicing
Cellular Component
GO:0005634 nucleus
GO:0005681 spliceosomal complex
GO:0005682 U5 snRNP
GO:0005737 cytoplasm
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071013 catalytic step 2 spliceosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:6bk8, PDBe:6bk8, PDBj:6bk8
PDBsum6bk8
PubMed29146870
UniProtP33334|PRP8_YEAST Pre-mRNA-splicing factor 8 (Gene Name=PRP8)

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