Structure of PDB 7ug7 Chain EF Binding Site BS04

Receptor Information

>7ug7 Chain EF (length=672) Species: 83333 (Escherichia coli K-12)

TTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKSAATTAFWSGMAKQ
YEPHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYCAVGGVQPQSETVWR
QANKYKVPRIAFVNKMDRMGANFLKVVNQIKTRLGANPVPLQLAIGAEEH
FTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEWHQNLIESAAE
ASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQAM
LDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDP
FVGNLTFFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVR
AGDIAAAIGLKDVTTGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQ
EKMGLALGRLAKEDPSFRVWTDEESNQTIIAGMGELHLDIIVDRMKREFN
VEANVGKPQVAYRETIRQKVTDVEGKHAKQSGGRGQYGHVVIDMYPLEPG
SNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPLAGYPVVDMGIRL
HFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETPEENT
GDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRA
SYTMEFLKYDEAPSNVAQAVIE

Ligand information

>7ug7 Chain mR (length=10)

gauguucaaa

..........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7ug7 The cyclic octapeptide antibiotic argyrin B inhibits translation by trapping EF-G on the ribosome during translocation.
• Resolution: 2.58 Å
• Binding residue (original residue number in PDB): G511 R512
• Binding residue (residue number reindexed from 1): G483 R484

Enzymatic activity

• Enzyme Commision number: 3.6.5.-

Gene Ontology

Molecular Function

• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding
• GO:0016787 hydrolase activity
• GO:0097216 guanosine tetraphosphate binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0032790 ribosome disassembly

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:7ug7, PDBe:7ug7, PDBj:7ug7
• PDBsum: 7ug7
• PubMed: 35500116
• UniProt: P0A6M8|EFG_ECOLI Elongation factor G (Gene Name=fusA)