Structure of PDB 6z6b Chain EEE Binding Site BS04

Receptor Information

>6z6b Chain EEE (length=2031) Species: 11577 (La Crosse virus)

GMDYQEYQQFLARINTARDACVAKDIDVDLLMARHDYFGRELCKSLNIEY
RNDVPFIDIILDIRPEVDPLTIDAPHITPDNYLYINNVLYIIDYKVSVSN
ESSVITYDKYYELTRDISDRLSIPIEIVIIRIDPVSRDLHINSDRFKELY
PTIVVDINFNQFFDLKQLLYEKFGDDEEFLLKVAHGDFTLTAPWCKTGCP
EFWKHPIYKEFKMSMPVPERRLFEESVKFNAYESERWNTNLVKIREYTKK
DYSEHISKSAKNIFLASGFYKQPNKNEISEGWTLMVERVQDQREISKSLH
DQKPSIHFIWGAHNPGNSNNATFKLILLSKSLQSIKGISTYTEAFKSLGK
MMDIGDKAIEYEEFCMSLKSKARSSWKQIMNKKLEPKQINNALVLWEQQF
MINNDLIDKSEKLKLFKNFCGIGKHSKPKILDFDDANMYLASLTMMEQSK
KILSKSNGLKPDNFILNEFGSRIKDANKETYDNMHKIFETGYWQCISDFS
TLMKNILSVSQYNRHNTFRIAMCANNNVFAIVFPSAATVVYSIIVLHKEE
ENIFNPGCLHGTFKCMNGYISISRAIRLDKERCQRIVSSPGLFLTTCLLF
KHDNPTLVMSDIMNFSIYTSLSITKSVLSLTEPARYMIMNSLAISSNVKD
YIAEKFSPYTKTLFSVYMTRLIKNACFDAYDQRQRVQLRDIYLSDYDITQ
KGIKDNRELTSIWFPGSVTLKEYLTQIYLPFYFNAKGLHEKHHVMVDLAK
TILEIECEQRENIKEIWSTNCTKQTVNLKILIHSLCKNLLADTSRHNHLR
NRIENRNNFRRSITTISTFTSSKSCLKIGDFRKEKELQSVKQKKILEVQS
RKMRLANPMFNYEMLRNAMPNYTDYISTKVFDRLYELLDKKVLTDKPVIE
QIMDMMIDHKKFYFTFFNKGQKTSKDREIFVGEYEAKMCMYAVERIAKER
CKLNPDEMISEPGDGKLKVLEQKSEQEIRFLVETTRQKNREIDEAIEALA
SNLGKIEKLSLGKAKGLKMEINADMSKWSAQDVFYKYFWLIALDPILYPQ
EKERILYFMCNYMDKELILPDELLFNLLDQKVAYQNDIIATMTNQLNSNT
VLIKRNWLQGNFNYTSSYVHSCAMSVYKEILKEAITLLDGSILVNSLVHS
DDNQTSITIVQDKMENDKIIDFAMKEFERACLTFGCQANMKKTYVTNCIK
EFVSLFNLYGEPFSIYGRFLLTSVGDCAYIGPYEDLASRISSAQTAIKHG
CPPSLAWVSIAISHWMTSLTYNMLPGQSNDPIDYFPAENRKDIPIELNGV
LDAPLSMISTVGLESGNLYFLIKLLSKYTPVMQKRESVVNQIAEVKNWKV
EDLTDNEIFRLKILRYLVLDAGRSILTPRKFTTAGSLRKLYSFSKYQDRL
SSPGGMVELFTYLLEKPELLVTKGEDMKDYMESVIFRYNSKRFKESLSIQ
NPAQLFIEQILFSHKPVIDFSGIRDKYINLGKVTFTEAYRLLMRDLSSLE
LTNDDIQVIYSYIILNDPMMITIANTHILSIYGSPQRRMGMSCSTMPEFR
SPALVLRAYSKNNPDTEMARDLVHLKEFVENTNLEEKMKVRIAMNEAEKG
QRDIVFELKEMTRFYQVCYEYVKSTEHKIKVFILPAKSYTTTDFCSLMQG
NLIKDKEWYTVHYLKQILSGGHKAINATSEQNIAFECFKLITHFADSFID
SLSRSAFLQLIIDEFSYKDVKVSKLYDIIKNGYNRTDFIPLLFRTGDLRQ
ADLDKYDAMKRILIRSLDYLNNDIFSLSRIKVGLDEFATIKKAHFSKMVS
FEGPPIKTGLLDLTELMKSQDLLNLNYDNIRNSNLISFSKLICCEGSDNI
NDGLEFLSDDPMNFTEGEAIHSTPIFNIYYSKRGERHMTYRNAIKLLIER
ETKIFEEAFTFSENGFISPENLGCLEAVVSLIKLLKTNEWSTVIDKCIHI
CLIKNGMDHMYHSFDVPKCFMGNPITRDINWVMFREFINSLPGTDIPPWN
VMTENFKKKCIALINSKFETQRDFSEFTKLM

Ligand information

• Ligand ID: ZN
• InChI: InChI=1S/Zn/q+2
• InChIKey: PTFCDOFLOPIGGS-UHFFFAOYSA-N
• SMILES:
  CACTVS 3.341: [Zn++]
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Zn+2]
• Formula: Zn
• Name: ZINC ION
• ChEMBL: CHEMBL1236970
• DrugBank: DB14532
• PDB chain: 6z6b Chain EEE Residue 2301

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6z6b Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes.
• Resolution: 3.961 Å
• Binding residue (original residue number in PDB): C2064 H2169 I2173 D2178
• Binding residue (residue number reindexed from 1): C1844 H1949 I1953 D1958
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016787 hydrolase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0044165 host cell endoplasmic reticulum
• GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
• GO:0044177 host cell Golgi apparatus
• GO:0044423 virion component

External links

• PDB: RCSB:6z6b, PDBe:6z6b, PDBj:6z6b
• PDBsum: 6z6b
• PubMed: 32681014
• UniProt: A5HC98|L_BUNLC RNA-directed RNA polymerase L (Gene Name=L)