Structure of PDB 8dh2 Chain E Binding Site BS04

Receptor Information
>8dh2 Chain E (length=841) Species: 10760 (Escherichia phage T7) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
INIAKNDFSDIELAAIPFNTLADHYGERLAREQLALEHESYEMGEARFRK
MFERQLKAGEVADNAAAKPLITTLLPKMIARINDWFEEVKAKRGKRPTAF
QFLQEIKPEAVAYITIKTTLACLTTTVQAVASAIGRAIEDEARFGRIRDL
EAKHFKKNVEEQLNKRVGHVYKKAFMQVVEADMLSKGLLGGEAWSSWHKE
DSIHVGVRCIEMLIESTGMVSLHRQNASETIELAPEYAEAIATRAGALAG
ISPMFQPCVVPPKPWTGITGGGYWANGRRPLALVRTHSKKALMRYEDVYM
PEVYKAINIAQNTAWKINKKVLAVANVITKWKHCPVEDIPAIAWKRAAAA
VYRKDKARKSRRISLEFMLEQANKFANHKAIWFPYNMDWRGRVYAVSMFN
PQGNDMTKGLLTLAKGKPIGKEGYYWLKIHGANCAGVDKVPFPERIKFIE
ENHENIMACAKSPLENTWWAEQDSPFCFLAFCFEYAGVQHHGLSYNCSLP
LAFDGSCSGIQHFSAMLRDEVGGRAVNLLPSETVQDIYGIVAKKVNEILQ
ADAINGTDNEVVTVTDENTGEISEKVKLGTKALAGQWLAYGVTRSVTKRS
VMTLAYGSKEFGFRQQVLEDTIQPAIDSGKGLMFTQPNQAAGYMAKLIWE
SVSVTVVAAVEAMNWLKSAAKLLAAEVKDKKTGEILRKRCAVHWVTPDGF
PVWQEYKKPIQTRLNLMFLGQFRDSEIDAHKQESGIAPNFVHSQDGSHLR
KTVVWAHEKYGIESFALIHDSFGTIPADAANLFKAVRETMVDTYESCDVL
ADFYDQFADQLHESQLDKMPALPAKGNLNLRDILESDFAFA
Ligand information
Ligand ID3PO
InChIInChI=1S/H5O10P3/c1-11(2,3)9-13(7,8)10-12(4,5)6/h(H,7,8)(H2,1,2,3)(H2,4,5,6)
InChIKeyUNXRWKVEANCORM-UHFFFAOYSA-N
SMILES
SoftwareSMILES
CACTVS 3.341O[P](O)(=O)O[P](O)(=O)O[P](O)(O)=O
OpenEye OEToolkits 1.5.0OP(=O)(O)OP(=O)(O)OP(=O)(O)O
ACDLabs 10.04O=P(OP(=O)(O)OP(=O)(O)O)(O)O
FormulaH5 O10 P3
NameTRIPHOSPHATE
ChEMBLCHEMBL1230191
DrugBankDB03896
ZINCZINC000006827739
PDB chain8dh2 Chain E Residue 901 [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
Receptor-Ligand Complex Structure
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PDB8dh2 Structural basis of transcription recognition of a hydrophobic unnatural base pair by T7 RNA polymerase.
Resolution2.9 Å
Binding residue
(original residue number in PDB)		K472 R627 K631 M635
Binding residue
(residue number reindexed from 1)		K439 R594 K598 M602
Annotation score3
Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0016779 nucleotidyltransferase activity
GO:0034062 5'-3' RNA polymerase activity
Biological Process
GO:0006351 DNA-templated transcription
GO:0019083 viral transcription
GO:0039695 DNA-templated viral transcription
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8dh2, PDBe:8dh2, PDBj:8dh2
PDBsum8dh2
PubMed36635281
UniProtP00573|RPOL_BPT7 T7 RNA polymerase (Gene Name=1)

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