Structure of PDB 6vzj Chain AA Binding Site BS04

Receptor Information

>6vzj Chain AA (length=1322) Species: 562 (Escherichia coli)

VYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEA
AFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLR
LVIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHR
SPGVFFDSDKGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRR
RKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQMELVPERLRGETA
SFDIEANGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKDY
IDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETL
RVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVG
RMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGNR
RIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAA
VKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVH
PTHYGRVCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTD
EIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVD
YMDVSTQQVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVG
TGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDEMYPGEAGIDIY
NLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQNMR
VAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEIT
ADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGSDVKDSSLRV
PNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAG
LFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDE
LKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRH
GNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMA
AKGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTFSDEEVM
RLAENLRKGMPIATPVFDGAKEAEIKELLKLGDLPTSGQIRLYDGRTGEQ
FERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRF
GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
ESFNVLLKEIRSLGINIELEDE

Ligand information

>6vzj Chain D (length=1524)

aaauugaagaguuugaucauggcucagauugaacgcuggcggcaggccua
acacaugcaagucgaacgguaacaggaagaagcuugcuucuuugcugacg
aguggcggacgggugaguaaugucugggaaacugccugauggagggggau
aacuacuggaaacgguagcuaauaccgcauaacgucgcaagaccaaagag
ggggaccuucgggccucuugccaucggaugugcccagaugggauuagcua
guaggugggguaacggcucaccuaggcgacgaucccuagcuggucugaga
ggaugaccagccacacuggaacugagacacgguccagacuccuacgggag
gcagcaguggggaauauugcacaaugggcgcaagccugaugcagccaugc
cgcguguaugaagaaggccuucggguuguaaaguacuuucagcggggagg
aagggaguaaaguuaauaccuuugcucauugacguuacccgcagaagaag
caccggcuaacuccggccagcagcccgguaauacggagggugcaagcguu
aaucggaauuacugggcguaaagcgcacgcaggcgguuuguuaagucaga
ugugaaauccccgggcucaaccugggaacugcaucugauacuggcaagcu
ugagucucguagagggggguagaauuccagguguagcggugaaaugcgua
gagaucuggaggaauaccgguggcgaaggcggcccccuggacgaagacug
acgcucaggugcgaaagcguggggagcaaacaggauuagauacccuggua
guccacgccguaaacgaugucgacuuggagguugugcccuugaggcgugg
cuuccggagcuaacgcguuaagucgaccgccuggggaguacggccgcaag
guuaaaacucaaaugaauugacgggggcccgcacaagcgguggagcaugu
gguuuaauucgauaacgcgaagaaccuuaccuggucuugacauccacgga
aguuuucagagaugagaaugugccuucgggaaccgugagacaggugcugc
auggcugucgucagcucguguugugaaauguuggguuaagucccgcaacg
agcgcaacccuuauccuuuguugccagcgguccggccgggaacucaaagg
agacugccagugauaaacuggaggaagguggggaugacgucaagucauca
ugcccuuacgaccagggcuacacacgugcuacaauggcgcauacaaagag
aagcgaccucgcgagagcaagcggaccucauaaagugcgucguaguccgg
auuggagucugcaacucgacuccaugaagucggaaucgcuaguaaucgug
gaucagaaugccacggugaauacguucccgggccuuguacacaccgccgu
acaccaugggaguggguugcaaaagaaguagguagcuuaaccuucgggag
ggcgcuuaccacuuugugauucaugacuggggugaagucgaacaagguaa
ccguaggggccugcgguuggauca

........<<<<..[.((((.>>>>.<<<<.<<<<<..<<<<<<<<....
.<<<.<<<..<<<..<<.<<..<<<<<<<<<......>>>>>>.>>>>>.
.>>>>>......<<.......<<<<<<<..<<...<<<<<<<.<.<<...
..<<<<<......>>>>>......>>.>.....<<<....>>>....<<<
<<<..<<....>>>>>>>>.>>>>>>>..>>.>>>>>>><<<....<<<.
.<<<<<<<.........>>>>>>>>>>......>>>..<<<<<<<<....
>>>>...>>>>.>>.<<<<<.<.........>>>>>>.<<<<....>>>>
...>>>>>>.........<<<....<<<<....>>>>..>>>..>>.>>>
>>>..<<<<......<<<<....>>>>.....>>>>..<<.<<<<<....
..<.<<<<<<<<.......>>>>>>>>.>........>>>>>....>>.<
<<<<(((...<<<<<.......)))........>>>>>>>>>>..>>>>>
>>>>..........<<<((.....<<<<...<<<.<<<<<<<.<<<<<<<
<<<......<<<<<<.....>>>>>>....>>>>>>>>..>>>>>>>>>.
..<<<<<<<<...<<<<<<<....<<<<<<<<...<<<......>>>...
...>>>>>>>>...........<<....>>.>>>>>>>..>>>>.>>>>.
..>>>...>>>>....<<<<<<...<<...<<<<.<.....>.>>>>...
>>>>>>>>..........<<<<<<.<<<<<<<<<<<<<.....>>>>>>>
>>>>>>..<<..))>>.....>>>>>>.>>>.<<<......<<<<....>
>>>....>>>..)))).]<<<<<.<<<<<<<.<<.<<<<<<..<<<<<<<
<<<......<<......>>..........<<<<<<<......<<<<<<<.
.<<<<<<<....>>>>>>>...<......>..>>>>>.>>.<<<......
<<<<<<.......<<<<<<<<<....>>>..<<<<......>>>>..>>>
>>>.....<<<<.<<<<<<<...<<..<<<.....>>>>>....>>>>>>
>.....<<<<<.....>>>>>........>>>>.........>>>...>>
>..>>>...>>>>>>>...>>.>>>>>>>>.....<<<<<<<.....<<<
..<<...<<<....>>>...>>....>>>.....>>>>>>>......<..
..<<<<<<<........>>>>>>>....>.....>>>>>>....<<<<<<
<.........>>>>>>>......>>...>>>>>>>>>>.>>......<<.
.<<<<.<<<..<<<<<<<<<<<<....<<<<<<.<<<<..<<<..>>>.>
>>>>>>>>>...>>>>>>>>>>>>..>>>.>>>>....>>........<<
<<<<<<<..>>>>>>>>>......

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6vzj Structural basis of transcription-translation coupling.
• Resolution: 4.1 Å
• Binding residue (original residue number in PDB): E1026 E1030
• Binding residue (residue number reindexed from 1): E1006 E1010

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0005515 protein binding
• GO:0016779 nucleotidyltransferase activity
• GO:0032549 ribonucleoside binding
• GO:0034062 5'-3' RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0006352 DNA-templated transcription initiation
• GO:0006879 intracellular iron ion homeostasis
• GO:0009408 response to heat
• GO:0031564 transcription antitermination
• GO:0032784 regulation of DNA-templated transcription elongation
• GO:0036460 cellular response to cell envelope stress
• GO:0042128 nitrate assimilation
• GO:0044780 bacterial-type flagellum assembly
• GO:0046677 response to antibiotic
• GO:0048870 cell motility
• GO:0071973 bacterial-type flagellum-dependent cell motility
• GO:0090605 submerged biofilm formation
• GO:2000142 regulation of DNA-templated transcription initiation

Cellular Component

• GO:0000345 cytosolic DNA-directed RNA polymerase complex
• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0008023 transcription elongation factor complex
• GO:0016020 membrane

External links

• PDB: RCSB:6vzj, PDBe:6vzj, PDBj:6vzj
• PDBsum: 6vzj
• PubMed: 32820061
• UniProt: P0A8V2|RPOB_ECOLI DNA-directed RNA polymerase subunit beta (Gene Name=rpoB)