Structure of PDB 7k58 Chain A Binding Site BS04

Receptor Information

>7k58 Chain A (length=4443) Species: 5911 (Tetrahymena thermophila)

KYSKRIAWMKTTICDSLQLKDMIVEESFQYEKNKNLLEQFLSGEGLNKIF
AYYQVQEQAQNTTGDLEKIQDKAVWFLRITNPADDKKKASQQDGNDNDII
FGEITPNTVPMLNALMESVYSRQIDHIITEKIQFAEEEQVLEFQQHSNKF
SSEVREAINLLDYEAISGLSESEKMQHYEMKFNEWINLISSQLRKDEKDA
GPATELIYWRSRMQKITNWSEQLKSKDFQIVKASLQRHKNHDNQRPRGDE
SKLNEAKDNVKYLTTLEKFIPLYNGTPQQIIDTLPALMNAIKMIHTTTDK
MTGLFIKITNQMIKNCKDRILNKMIWEQDPAELIEVLGSCIKLYCEYKKC
YNDTKEKVADMPKGKTSDAQIFGKFDTFVRRLQKLIEIFSNIQQFNALLE
GMDVLTNKFKKIIDDFKKKGHNLLDTANNKFDRDWVEFNVEISHLDGELQ
NFIDNNFNRFREYSLKLLHKFQSTIKRHNLTSRYNAILHNYATELDTIQR
VFQDQKSNPPLVRNMPPEAGKIIWARHLFQKITGPINTEIRRYYGSYNTL
GKQLTIYEMWFYQDWVNKIEQSKAALQATLIVRHDENKKLYVNFDLEIMQ
LIREAKCLDRQGIEIPESARIILLQEDKFKTYYNELLYALKEYERINSKI
KPICKNLLLPHIEDLDLKLRPGMVTLTWTSMNIESYLYYVHQGLKKLEQL
IINVNDIIENRIENNLKTVSKVVLVHLPLDSFVQLQEEYINSKTDFLTSK
NVEVERAVDDLLQTIMLYPLDPHVDPVLPEETKRIKRYYFWYFYQALLNS
TQNSLNAMKYRFFQVEVQLNGDKVTLIQKSINRAATAVLRCSKHLYNWDQ
ATFYDMIACDKEIVKVILLLTGSIQGTKNKVNEFLSGFTKFEWLCKESIQ
ESIKKFSKNGPTLQNYEDQLKKFSQIEEEIEKIVPTYKIGAMELMTHNIC
TSLSTWAKEWKLQYSQDLHKRARQLLDSLTEQTKMLSTKLSKPVKDIDSL
GYVMETLEQIRKEQAEIDMKFNPVQEMYSLLDNYLPGGITDKDEMDARSL
LRRNWDILIQQAEIKGKEYQHKQAIYLKELKQSIKDFTNQVSIFRRDYEK
NGPMERLRRFEDEYDVKYQMYKINARGENLFGLQNQKYPELEKTDAEIKN
LNKLYNLYDSVIKNIQQFKEKSWQDVSLAKMEEDAGKYGEQCSRLPKDLK
EWQAYRDLKNYIDSLREQLPLIIPRHWEKIKEITNTKLNYENPDQFYIEE
IMGALDFREDIEDITESADKQLKIRTGLDEINLYWNDMQFQFGIWGKRML
NGLIVGTILERLEEDQLQLSTFNSQRHVTPFKAEVENLIRTFSDVNDTLD
MWVKVQKLWTSLEPVFTGGDIARQMPLQAKQFQGIDKNWMKIMEKAVETK
KVIPCCQNDMLKDFLPDLNRKLEDCQKMLEAYLEGKRKKFPRFYFVSNPT
LLKILSQGSEPTSIQEDFEKLFDAITKVTFESAKDKKNPALKQITQIQQV
IGRNEENISLTGYYVKCEGNIEDWLKKLEQNMQQTLKDIASAAAQQVFQV
GLKEFVSSQASQIALLGLQILWTSKVNEGLERLSRNERNAMDIKRNEIKE
HMNILSSMCLEDLNGAVERTKVETLVTIQVHQKDISMDLKCKDVNDFEWQ
KQTRIAWKTDIDECIISITDWDSPYSYEFLGAKERLCITPLTDRCYITLA
QAMSMYYGGAPAGPAGTGKTETVKDLGRTLGVFVVVTNCSDQHRYRDMAK
IFKGLVQSGLWGCFDEFNRIDLEVLSVVAMQVESITTARKQHMKKFMFPE
EEIEIELIPTVSYFITMNPGYAGRQELPENLKVLFRGVSMMVPDREIIIK
VKLASVGYLQIDLLAKKFNVLYRLCEEQLSKQRHYDFGLRNILSVLRTAG
NTKRQEIKSDEEMLLMRSLRDMNLSKLVADDIPLFNGLLADIFPKLKEVP
KKLYPDVEKKIPEEINAESYLINTPSFQLKIIQLYETCLVRHGFMLVGPT
GSGKSTIMKILTEVLTKLGSPHKIVIMNPKAITAEQMYGVKSEISDDWIP
GVFSTIWAKSNNRALKHTTWITCDGPVDAIWIENLNTVLDDNKILTLANG
ERIAMTENCKVVFEVENLNNASPATVSRCGQVYVSPTDLGYEAVIEGWIR
NRKASGRAEESDKLGNILRKYLINMRFIELQSKECKEPMMDTSPVISVIN
ILNLLTGCLQYFVQTQRTLSEQEYEKFIVYSMAWAIGGIYEAQDRVRFHE
LLLAKNAPIPQKGKENETVFDYYVSQDYLDWKICSPEEWVPPQSLQFSQL
LLPTLDSFRAEMLLNFILTQPKSHTCSNSALLIGGSGTAKTSSVLLYCNK
FDPQKMLFKRTNFSSATSPFMFQSTIEAECDFKVGKEFAPPGNKMMTIFI
DDMSMPFVNKWGDQITLELVRQLIETGGFYMLDKTQRGNQRKMKNLQYIG
AMNHPGGGRNDIPNRLKRQFFIFNMILPLSIEGIYGPIIKHMFKQKYFSD
STYKVIESLTSATIALWNKVKSTMLPTPAKFHYVFNMRELSRIFKGILTC
KKDTINDAPKSMKIKPELFLVGLWRHEAERVLADKLVNNKDKDTVMGYIQ
EVSLESFSQIENEILEKYSSEKTFLFCDFLRPDVINEDGIIEEEAPKIYE
AIDSLTELRKRCNFLLSFYNDRNPSKKMPLVLFDDALKHLLRISRIIRQP
RSSGLLVGVGGSGKQSLTRLAGFIGKNLIQQIIVTKTYSDKDLKEDIKKG
FDDAGHLGKQVTFLMTDSEVKKEEFLEYINMVLSTGEIPNLLAKDEREVW
LGDISQAYCKEKNLGNIDPPQSELWTYFVDRVRDNFHIMLCFSPVGQKFR
ERARKFPALFNECTIDWFLPWPEEALVSVAETFIKNFDKLDTKEETKQEL
MKHMGNVHLMVNEICDEYYQKMRRQVYVTPKSFLSYLNSYKTLYIEKYDE
LDQQEESFKIGLNKIQEATITINQMEISLKEEEIQLNEATEKTNQLLANL
DKESKKANQKGEEVAATNKQCEIQAEQISKEKEEAERELEAALPALRRAQ
EAVDSIESKDIVELKANKKPLDIIKYIMDAVLVFFKARLIPIQIEERVFN
KKEGKAVLFLKESYDESGIQTLGDMNFMKKLKEFEKDSINEETIELLEPY
LNQSEDWFNDTFATKASKAAAGILKWAFAIYEYHQKSKIVKPKRIQVAIA
EGRQAIALKELEKAREDLAQIQAYIKNLKDVYTKQMEEKNELEMKAAKTK
KKINTARTLITSLSGEKDRWGKGAQDISDQKRKLVGNVSLSTAFISYCGP
FNAEYRNKLAQQRFVVDMKKRGVPVTPGLELTSFLIDDATIGEWNLQGLP
KDDLSIQNGIMVTNSARYPLFIDPQGQGQNWIRNKLSASIIPERCITTLS
HPKFKDMFLKYCMESGLTLIVENIENEVDPMMDPVLERQIIVKGKTQFVN
VAGTEMELSKEFKLFMTCRLANPSFSPELSAKTTIIDFTVTQSGLEQQLL
GKVISKEQKALEDSLNQLLADVNQNQKDLQRLDKNLLERLINSQGNLLDD
TELMDVLNNTKTQAKEVAAKLIDAEIKTKEINEKREQYRPVAIRGSAIYF
TMIEVSLVNWMYNSSLEQFLKLFIESIDLSEKAQLPSNRVKNIISFLTFH
VYRYVNRGLFEKDKITFILMMAFKILTTAGTISSGDVSLFLKSGDALDIK
SERQKQISYLEDNQWLNILALSKHTFSGQTLPFFKELPDLISRSENQWRN
WIDKNDPENFPIPDFAESINQEKEIGSFISLCLVRSLRNDRTLIATQNFI
SNVLGKEFTDPISYPIEGIWQESSNMDPVLFLLSAGADPTSSIDELAKKK
KKFPCEKVSMGEGQERVARQVIMKGFVEGGWVILQNCHLGLKFMEEIETL
VSPINQIHEDFRLWITCEQHPKFPLGLLQKTLKVTNEPPKGLKAGLYKTF
TTIITQEFIDKVDHSNWRSLIFTICFLHSIVIERKKFGPLGWCVPYEYNY
SDLEASLLYIEKYLTNLMSTPHNLPISMNVVRYMICEVQYGGRITDDLDR
ELFITYGETYLKDGIFGNDYFFYDIMVDQKFKYRIPQNPSAELIKYQEYI
AKVPTVDNPEVFGLHSNADLTFRLKESKEMINTVMETRPKDSSVGGGKTR
EEIVQDKAKDMLKNLPPDYNDVEVRELVSKLGGPNPKTSTERGMTVPLNI
FLYQEVTRMQRVIGLVRKTLQDTILAIDGQIIMTPEILEAINAIYDAKVP
NSWLYDPSGAEISWLLPNLGSWSTSLSDRNKQLNDWLRSGQRPILFWLTG
FFNPQGFLTGMKQEVTRNHKGKGGEAWSLDDVVYSTTVKEREKEKDIEQP
PAEGVYIKGLYLEGCKWSKNGLDDSDPKKIFADLPILHVSAINKKMSNTY
LCPVYKYPKRTDKYLIFRVGLPCEGSNNPSHWKLRGVALLCST

Ligand information

• Ligand ID: MG
• InChI: InChI=1S/Mg/q+2
• InChIKey: JLVVSXFLKOJNIY-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mg+2]
  CACTVS 3.341: [Mg++]
• Formula: Mg
• Name: MAGNESIUM ION
• DrugBank: DB01378
• PDB chain: 7k58 Chain A Residue 5001

Receptor-Ligand Complex Structure

Structure summary

• PDB: 7k58 Structures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism.
• Resolution: 4.0 Å
• Binding residue (original residue number in PDB): S2164 E2273
• Binding residue (residue number reindexed from 1): S2005 E2114
• Annotation score: 1

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008569 minus-end-directed microtubule motor activity
• GO:0016887 ATP hydrolysis activity
• GO:0045505 dynein intermediate chain binding
• GO:0051959 dynein light intermediate chain binding

Biological Process

• GO:0007018 microtubule-based movement

Cellular Component

• GO:0005737 cytoplasm
• GO:0005856 cytoskeleton
• GO:0005858 axonemal dynein complex
• GO:0005874 microtubule
• GO:0005929 cilium
• GO:0030286 dynein complex

External links

• PDB: RCSB:7k58, PDBe:7k58, PDBj:7k58
• PDBsum: 7k58
• PubMed: 34556869
• UniProt: Q22A67