Structure of PDB 6z8k Chain A Binding Site BS04

Receptor Information

>6z8k Chain A (length=2158)

MDYQEYQQFLARINTARDACVAKDIDVDLLMARHDYFGRELCKSLNIEYR
NDVPFIDIILDIRPEVDPLTIDAPHITPDNYLYINNVLYIIDYKVSVSNE
SSVITYDKYYELTRDISDRLSIPIEIVIIRIDPVSRDLHINSDRFKELYP
TIVVDINFNQFFDLKQLLYEKFGDDEEFLLKVAHGDFTLTAPWCKTGCPE
FWKHPIYKEFKMSMPVPERRLFEESVKFNAYESERWNTNLVKIREYTKKD
YSEHISKSAKNIFLASGFYKQPNKNEISEGWTLMVERVQDQREISKSLHD
QKPSIHFIWGAHNPGNSNNATFKLILLSKSLQSIKGISTYTEAFKSLGKM
MDIGDKAIEYEEFCMSLKSKARSSWKQIMNKKLEPKQINNALVLWEQQFM
INNDLIDKSEKLKLFKNFCGIGKSKPKILDFDDANMYLASLTMMEQSKKI
LSKSNGLKPDNFILNEFGSRIKDANKETYDNMHKIFETGYWQCISDFSTL
MKNILSVSQYNRHNTFRIAMCANNNVFAIVFPATVVYSIIVLHKEEENIF
NPGCLHGTFKCMNGYISISRAIRLDKERCQRIVSSPGLFLTTCLLFKHDN
PTLVMSDIMNFSIYTSLSITKSVLSLTEPARYMIMNSLAISSNVKDYIAE
KFSPYTKTLFSVYMTRLIKNACFDAYDQRQRVQLRDIYLSDYDITQKGIK
DNRELTSIWFPGSVTLKEYLTQIYLPFYFNAKGLHEKHHVMVDLAKTILE
IECEQRENIKEIWSTNCTKQTVNLKILIHSLCKNLLADTSRHNHLRNRIE
NRNNFRRSITTISTFTSSKSCLKIGDFRKEKELQSVKQKKILEVQSRKNY
EMLRNAMPNYTDYISTKVFDRLYELLDKKVLTDKPVIEQIMDMMIDHKKF
YFTFFNKGQKTSKDREIFVGEYEAKMCMYAVERIAKERCKLNPDEMISEP
GDGKLKVLEQKSEQEIRFLVETTRQKNREIDEAIELGKIEKLSLGKAKGL
KMEINADMSKWSAQDVFYKYFWLIALDPILYPQEKERILYFMCNYMDKEL
ILPDELLFNLLDQKVAYQNDIIATMTNQLNSNTVLIKRNWLQGNFNYTSS
YVHSCAMSVYKEILKEAITLLDGSILVNSLVHSDDNQTSITIVQDKMEND
KIIDFAMKEFERACLTFGCQANMKKTYVTNCIKEFVSLFNLYGEPFSIYG
RFLLTSVGDCAYIGPYEDLASRISSAQTAIKHGCPPSLAWVSIAISHWMT
SLTYNMLPGQSNDPIDYFPAENRKDIPIELNGVLDAPLSMISTVGLESGN
LYFLIKLLSKYTPVMQKRESVVNQIAEVKNWKVEDLTDNEIFRLKILRYL
VLDAEMDPSDIMGETSDMRGRSILTPRKFTTAGSLRKLYSFSKYQDRLSS
PGGMVELFTYLLEKPELLVTKGEDMKDYMESVIFRYNSKRFKESLSIQNP
AQLFIEQILFSHKPVIDFSGIRDKYINLGKVTFTEAYRLLMRDLSSLELT
NDDIQVIYSYIILNDPMMITIANTHILSIYGSPQRRMGMSCSTMPEFRNL
KLIHHSPALVLRAYSKNNPDPTEMARDLVHLKEFVENTNLEEKMKVRIAM
NEAEKGQRDIVFELKEMTRFYQVCYEYVKSHKIKVFILPAKSYTTTDFCS
LMQGNLIKDKEWYTVHYLKQILSGGHKAIMQHNATSEQNIAFECFKLITH
FADSFIDSLSRSAFLQLIIDEFSYKDVKVSKLYDIIKNGYNRTDFIPLLF
RTGDLRQADLDKYDAMKSHERVTWNDWINLTITGYNRSITIIGEDNKLTY
AELCLTRKTPENITISGRKLLGSRHGLKFENMSKIQNYYITYRKKDRHQF
VYQIHSHESITRRNEEHMAIRTRIYNEITPVCVVNVAEVDGDQRILIRSL
DYLNNDIFSLSRIKVGLDEFATIKKAHFSKMVSFEGPPIKTGLLDLTELM
KSQDLLNLNYDNIRNSNLISFSKLICCEGSDNINDGLEFLSDDPMNFTEG
EAIHSTPIFNIYYSKRGERHMTYRNAIKLLIERETKIFEEAFTFSENGFI
SPENLGCLEAVVSLIKLLKTNEWSTVIDKCIHICLIKNGMDHMYHSFDVP
KCFMGNPITRDINWVMFREFINSLPGTDIPPWNVMTENFKKKCIALINSK
FSEFTKLM

Ligand information

>6z8k Chain P (length=10)

guacacuacu

..........

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6z8k Pre-initiation and elongation structures of full-length La Crosse virus polymerase reveal functionally important conformational changes.
• Resolution: 3.02 Å
• Binding residue (original residue number in PDB): Y750 S840 R958 Q1145 G1146 H1185 S1186 D1187 V1239 S1240 R1254 D1262 A1264 V1472 K1474 S1497
• Binding residue (residue number reindexed from 1): Y728 S818 R915 Q1092 G1093 H1132 S1133 D1134 V1186 S1187 R1201 D1209 A1211 V1419 K1421 S1444

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003723 RNA binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016787 hydrolase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0044165 host cell endoplasmic reticulum
• GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
• GO:0044177 host cell Golgi apparatus
• GO:0044423 virion component

External links

• PDB: RCSB:6z8k, PDBe:6z8k, PDBj:6z8k
• PDBsum: 6z8k
• PubMed: 32681014
• UniProt: A5HC98|L_BUNLC RNA-directed RNA polymerase L (Gene Name=L)