Structure of PDB 6tay Chain A Binding Site BS04

Receptor Information

>6tay Chain A (length=4417) Species: 10090 (Mus musculus)

AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAKLTLGLSILFMVEAAEFTVPKKDLDSLCYLLIP
SAGSPEALHSDLSPVLRIRQRWRIYLTNLCLRCIDERCDRWLGILPLLHT
CMQKSPPKKNSKSQPEDTWAGLEGISFSEFRDKAPTRSQPLQFMQSKMAL
LRVDEYLFRSWLSVVPLESLSSYLENSIDYLSDVPVRVLDCLQGISYRLP
GLRKISNQNMKKDVENVFKMLMHLVDIYQHRIFGENLLQIYLTECLTLHE
TVCNITANHQFFEIPALSAELICKLLELSPPGEKSYEDLVTSTLQEALAT
TRNWLRSLFKSRMLSISSAYVRLTYSEEMAVWRRLVEIGFPEKHGWKGSL
LGDMEGRLKQEPPRLQISFFCSSQCRDGGLHDSVSRSFEKCVIEAVSSAC
QSQTSVLEGLSCQDLQKFGTLLSAVITKSWPVHNGEPVFDVDEIFKYLLK
WPDVRQLFELCGTNEKIIDNITEEGRQLMATAESVFQKVAGELENGTIVV
GQLELILEHQSQFLDIWNLNRRRDVRSLLKRRRDDLLFLKQEKRYVESLL
RQLGRVKHLVQVDFGNIEIIHSQDLSNKKLNEAVIKLSYKRETHYCLSPD
IREMASKLDSLKDSHIFQDFWQETAESLNELKVSLPEVLEYLYNPCYDNF
YTLYENLKSGKITFAEVDAIFKDFVDKYDELKNDLKFMCTMNPQDQKGWI
SERVGQIKEYHTLHQAVSSAKVILQVRRALGVTGDFSVLNPLLNFADSFE
DFGNESPQFIKAKQLLQDISEPRQRCLEELARQTELVAWLHKALEDINEL
KVFVDLASISAGENDIDVDRVACFHDAVQGYASLLYKMDERTNFSDFMNH
LQELWRALDNDQHLPDKLKDSARNLEWLKTVKESHGSVELSSLSLATAIN
SRGVYVIEAPKDGQKISPDTVLRLLLPDPEALRTYSTEELKELLNKLMLM
SGKKDHNSNTEVEKFSEVFSNMQRLVHVFIKLHCAGNMLFRTWTAKVYCC
PDGGIFMNFGLELLSQLTEKGDVIQLLGALCRQMEDFLDNWKTVVAQKRA
EHFYLNFYTAEQLVYLSSELRKPRPSEAALMMLSFIKGKCTVQDLVQATS
ACESKADRYCLREVMKKLPQQLLSEPSLMGKLQVIMMQSLVYMSAFLPHC
LDLDALGRCLAHLATMGGTPVERPLPKGLQAGQPNLILCGHSEVLPAALA
IYMQAPRQPLPTFDEVLLCTPATTIEEVELLLRRCLTSGSQGHKVYSLLF
ADQLSYEVGCQAEEFFQSLCTRAHREDYQLVILCDAAREHCYIPSTFSQY
KVPLVPQAPLPNIQAYLQSHYQVPKRLLSAATVFRDGLCVGIVTSERAGV
GKSLYVNTLHTKLKAKLRDETVPLKIIRLTEPHLDENQVLSALLPFLKEK
YQKMPVIFHIDISTSVQTGIPIFLFKLLILQYLMDINGKIWRRSPGHLYL
VEIPQPLFKFLDLFPKVTCRPPKEVIDMEPAMDPVEFCSEAFQRPYQYLK
RFHQQQNLDTFQYEKGSVEGSPEECLQHFLIYCGLINPSWSELRNFAWFL
NCQLKDCEASIFCKSAFTGDTLRGFKNFVVTFMILMARDFATPTEEDLAP
FSLRKRWESEPHPYVFFNGDHMTMTFIGFHLETNNNGYVDAINPSNGKVI
KKDVMTKELFDGLRLQRVPFNIDFDNLPRYEKLERLCLALGIEWPIDPDE
TYELTTDNMLKILAIEMRFRCGIPVIIMGETGCGKTRLIKFLSDLKRGSV
EAETMKLVKVHGGTTPSMIYSKVKEAERTAFSNKAQHKLDTILFFDEANT
TEAVSCIKEILCDRTVDGEHLHEDSGLHIIAACNPYRKHSQEMILRLESA
GLGYRVSAEETADRLGSIPLRQLVYRVHALPPSLIPLVWDFGQLNDSAEK
LYIQQIVQRLVDSVSVNPSETCVIADVLSASQMFMRKRENECGFVSLRDV
ERCVKVFRWFHDHSDMLLKELDKFLHESSDSTHTFERDPVLWSLVMAIGV
CYHASLEEKASYRTAIARCFPKPYNSSRAILDEVTHVQDLFLRGAPIRTN
IARNLALKENVFMMVICIELKIPLFLVGKPGSSKSLAKIIVADAMQGQAA
FSELFRCLKQVHLVSFQCSPHSTPQGIISTFKQCARFQQGKDLGQYVSVV
VLDEVGLAEDSPKMPLKTLHPLLEDGCIEDDPAPYKKVGFVGISNWALDP
AKMNRGIFVSRGSPNEKELIESAEGICSSDRLVQDKIRGYFAPFAKAYET
VCQKQDKEFFGLRDYYSLIKMVFAKAKASKRGLSPQDITHAVLRNFSGKD
NIQALSIFTASLPEARYKEEVSTVELIKQNIYPGPGAESRYLLVLTRNYV
ALQILQQTFFEGQQPEIIFGSSFPQDQEYTQICRNINRVKICMETGKMVV
LLNLQNLYESLYDALNQYYVYLGGQKYVDLGLGTHRVKCRVHTAFRLIVI
EEKDVVYKQFPVPLINRLEKHYLDMNTVLQPWQKSIVQELQQWAHEFADV
KADQFIARHKYSPADVFIGYHSDACASVVLQAVERQGCRDLTEELYRKVS
EEARSILLDCATPDAVVRLSGSSLGSFTAKQLSQEYYYAQQHNSFVDFLQ
AHLRMTHHECRAVFTEITTFSRLLTGNDCDVLASELRGLASKPVVLSLQQ
YDTEYSFLKDVRSWLTNPGKRKVLVIQADFDDGTRSAQLVASAKYTAINE
INKTQGTKDFVFVYFVTKLSRMGSGTSYVGFHGGLWRSVHIDDLRRSTIM
ASDVTKLQNVTISQLFKPESQFLDTTRLVQSCVQGAVGMLRDQNESCARN
MRRVTILLDLLNEDNTRNASFLRESKMRLHVLLNKQEENQVRSLKEWVTR
EAANQDALQEAGTFRHTLWKRVQDVVTPILASMIAHIDRDGNLELLAQPD
SPAWVQDLWMFIYSDIKFLNISLVMSFILVQSHMNLLKDAYNAVPFSWRI
RDYLEELWVQAQYITDTEGLSKKFVEIFQKTPLGVFLAQFPVAQQQKLLQ
SYLKDFLLLTMKVSSREELMFLQMALWSCLRELQEASGTPDETYKFPLSL
PWVHLAFQHFRTRLQNFSRILTIHPQVLSSLSQAAEKHSLAGCEMTLDAF
AAMACAEMLKGDLLKPSPKAWLQLVKNLSTPLELVCSEGYLCDSGSMTRS
VIQEVRALWNRIFSIALFVEHVLLGTESHIPELSPLVTTYVSLLDKCLEE
DSNLKTCRPFVAVMTTLCDCKDKASKKFSRFGIQPCFICHGDAQDPVCLP
CDHVYCLRCIQTWLIPGQMMCPYCLTDLPDKFSPTVSQDHRKAIEKHAQF
RHMCNSFFVDLVSTMCFKDNTPPEKSVIDTLLSLLFVQKELLHREHTKSL
SPFDDVVDQTPVIRSVLLKLLLKYSFHEVKDYIQNYLTQLEKKAFLTEDK
TELYLLFISCLEDSVHQKTSAGCRNLEQVLREEGHFLRTYSPGLQGQEPV
RIASVEYLQEVARVRLCLDLAADFLSELQEGSELAEDKRRFLKHVEEFCT
RVNNDWHRVYLVRKLSSQRGMEFVQSFSKQGHPCQWVFPRKVIAQQKDHV
SLMDRYLVHGNEYKAVRDATAKAVLECKTLDIGNALMACRSPKPQQTAYL
LLALYTEVAALYRSPNGSLHPEAKQLEAVNKFIKESKILSDPNIRCFARS
LVDNTLPLLKIRSANSILKGTVTEMAVHVATILLCGHNQILKPLRNLAFY
PVNMANAFLPTMPEDTQTGHVLGSPQSSGVAEVSDRGQSPVVFILTRLLT
HLAMLVGATHNPQALTVIIKPWVQDPQGFLQQHIQRDLEQLTKMLGRSAD
ETIHVVHLILSSLLRVQSHGVLNFNAELSTKGCRNNWEKHFETLLLRELK
HLDKNLPAINALISQDERISSNPVTKIIYGDPATFLPHLPQKSIIHCSKI
WSCRRKITVEYLQHIVEQKNGKETVPVLWHFLQKEAELRLVKFLPEILAL
QRDLVKQFQNVSRVEYSSIKGFIHSHSSDGLRKLLHDRITIFLSTWNALR
RSLETNGEIKLPKDYCCSDLDLDAEFEVILPRRQGLGLCGTALVSYLISL
HNNMVYTVQKFSNEDNSYSVDISEVADLHVISYEVERDLNPLILSNCQYQ
VQQGGETSQEFDLEKIQRQISSRFLQGKPRLTLKGIPTLVYRRDWNYEHL
FMDIKNKMAQSSLPNLAISTISGQLQSYSDACEALSIIEITLGFLSTAGG
DPGMDLNVYIEEVLRMCDQTAQVLKAFSRCQLRHIIALWQFLSAHKSEQR
LRLNKELFREIDVQYKEELSTQHQRLLGTFLNEAGLDAFLLELHEMIVLK
LKGPRAANSFNPNWSLKDTLVSYMETKDSDILSEVESQFPEEILMSSCIS
VWKIAATRKWDRQSRGG

Ligand information

• Ligand ID: ZN
• InChI: InChI=1S/Zn/q+2
• InChIKey: PTFCDOFLOPIGGS-UHFFFAOYSA-N
• SMILES:
  CACTVS 3.341: [Zn++]
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Zn+2]
• Formula: Zn
• Name: ZINC ION
• ChEMBL: CHEMBL1236970
• DrugBank: DB14532
• PDB chain: 6tay Chain A Residue 5204

Receptor-Ligand Complex Structure

Structure summary

• PDB: 6tay Moyamoya disease factor RNF213 is a giant E3 ligase with a dynein-like core and a distinct ubiquitin-transfer mechanism.
• Resolution: 3.2 Å
• Binding residue (original residue number in PDB): C3947 C3950 C3967 C3970
• Binding residue (residue number reindexed from 1): C3286 C3289 C3306 C3309
• Annotation score: 1

Enzymatic activity

• Enzyme Commision number: 2.3.2.-
  2.3.2.27: RING-type E3 ubiquitin transferase.
  3.6.4.-

Gene Ontology

Molecular Function

• GO:0004842 ubiquitin-protein transferase activity
• GO:0016887 ATP hydrolysis activity
• GO:0046872 metal ion binding

External links

• PDB: RCSB:6tay, PDBe:6tay, PDBj:6tay
• PDBsum: 6tay
• PubMed: 32573437
• UniProt: E9Q555|RN213_MOUSE E3 ubiquitin-protein ligase RNF213 (Gene Name=Rnf213)