Structure of PDB 5mq0 Chain A Binding Site BS04

Receptor Information
>5mq0 Chain A (length=1914) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence] [Download receptor structure] [Download structure with residue number starting from 1] [View receptor structure]
LYTPKAEMPPEHLRKIINTHSDMASKMYNTDKKAFLGALKYLPHAILKLL
ENMPHPWEQAKEVKVLYHTSGAITFVNETPRVIEPVYTAQWSATWIAMRR
EKRDRTHFKRMRFPPFDDDEPPLSYEQHIENIEPLDPINLPLDSQDDEYV
KDWLYDSRPLEEDSKKVNGTSYKKWSFDLPEMSNLYRLSTPLRDEVTDKN
YYYLFDKKSFFNGKALNNAIPGGPKFEPLYPFNSIDRVIFRVPIRSEYKV
AFPHLYNSRPRSVRIPWYNNPVSCIIQNDEEYDTPALFFDPSLEDFAPLL
AEEEELILPNTKDAMSLYHSPFPFNRTKGKMVRAQDVALAKKWFLQHPDE
EYPVKVKVSYQKLLKNYVLNELHPTLPTNHNKTKLLKSLKNTKYFQQTTI
DWVEAGLQLCRQGHNMLNLLIHRKGLTYLHLDYNFNLKPTKTLTTKERKK
SRLGNSFHLMRELLKMMKLIVDTHVQFRLGNVDAFQLADGIHYILNHIGQ
LTGIYRYKYKVMHQIRACKDLKHIIYYKFNKNLGKGPGCGFWQPAWRVWL
NFLRGTIPLLERYIGNLITRQFEGRSNEIVKTTTKQRLDAYYDLELRNSV
MDDILEMMPESIRQKKARTILQHLSEAWRCWKANIPWDVPGMPAPIKKII
ERYIKSKADAWVSAAHYNRERIKRGAHVEKTMVKKNLGRLTRLWIKNEQE
RQRQIQKNGPEITPEEATTIFSVMVEWLESRSFSPIPFPPLTYKNDTKIL
VLALEDLKDVYASKVRLNASEREELALIEEAYDNPHDTLNRIKKYLLTQR
VFKPVDITMMENYQNISPVYSVDPLEKITDAYLDQYLWYEADQRKLFPNW
IKPSDSEIPPLLVYKWTQGINNLSEIWDVSRGQSAVLLETTLGEMAEKID
FTLLNRLLRLIVDPNIADYITAKNNVVINFKDMSHVNKYGLIRGLKFASF
IFQYYGLVIDLLLLGQERATDLAGPANNPNEFMQFKSKEVEKAHPIRLYT
RYLDRIYMLFHFEEDEGEELTDEYLAENPDPNFENSIGYNNRKCWPKDSR
MRLIRQDVNLGRAVFWEIQSRVPTSLTSIKWENAFVSVYSKNNPNLLFSM
CGFEVRILPRQRMEEVVSNDEGVWDLVDERTKQRTAKAYLKVSEEEIKKF
DSRIRGILMASGSTTFTKVAAKWNTSLISLFTYFREAIVATEPLLDILVK
GETRIQNRVKLGLNSKMPTRFPPAVFYTPKELGGLGMISASHILIPASDL
SWSKQTDTGITHFRAGMTHEDEKLIPTIFRYITTWENEFLDSQRVWAEYA
TKRQEAIQQNRRLAFEELEGSWDRGIPRISTLFQRDRHTLAYDRGHRIRR
EFKQYSLERNSPFWWTNSHHDGKLWNLNAYRTDVIQALGGIETILEHTLF
KGTGFNSWEGLFWQIPNRRFTLWWSPTINRANVYVGFLVQLDLTGIFLHG
KIPTLKISLIQIFRAHLWQKIHESIVFDICQILDGELDVLQIESVTKETV
HPRKSYKMNSSAADITMESVHEWEVSKPSLLHETNDSFKGLITNKMWFDV
QLRYGDYDSHDISRYVRAKFLDYTTDNVSMYPSPTGVMIGIDLAYNMYDA
YGNWFNGLKPLIQNSMRTIMKANPALYVLRERIRKGLQIYQSSVQEPFLN
SSNYAELFNNDIKLFVDDTNVYRVTVHKTFEGNVATKAINGCIFTLNPKT
GHLFLKIIHTSVWAGQKRLSQLAKWKTAEEVSALVRSLPKEEQPKQIIVT
RKAMLDPLEVHMLDFPNIAIRPTELRLPFSAAMSIDKLSDVVMKATEPQM
VLFNIYDDWLDRISSYTAFSRLTLLLRALKTNEESAKMILLSDPTITIKS
YHLWPSFTDEQWITIESQMRDLILTEYGRKYNVNISALTQTEIKDIILGQ
NIKAPSVKRQKMAE
Ligand information
>5mq0 Chain 6 (length=99) [Search RNA sequence] [Download ligand structure] [Download structure with residue number starting from 1] [View ligand structure]
guucgcgaagcuucguggacauuuggucaauuugaaacaauacagagaug
aucagcaguuccccugcauaaggaugaaccguuuuacaaagagauuuau
<<<<<<<<<..>>>>>>>>>..............................
.......<<<..<<<.....>>>...>>>....................
Receptor-Ligand Complex Structure
Global viewLocal viewStructure summary

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PDB5mq0 Structure of a spliceosome remodelled for exon ligation.
Resolution4.17 Å
Binding residue
(original residue number in PDB)		K152 M153 K555 K586 T606 K611 G616 S618 Y725 N728 L729 R732 R737 I741 K743 T744 T746 Q748 R749 A752 Y753 H1863 T1865 E1867 N1869 A1871 K1873 A1900 G1901 K1903
Binding residue
(residue number reindexed from 1)		K26 M27 K393 K424 T444 K449 G454 S456 Y563 N566 L567 R570 R575 I579 K581 T582 T584 Q586 R587 A590 Y591 H1677 T1679 E1681 N1683 A1685 K1687 A1714 G1715 K1717
Enzymatic activity
Enzyme Commision number ?
Gene Ontology
Molecular Function
GO:0000386 second spliceosomal transesterification activity
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0017070 U6 snRNA binding
GO:0030619 U1 snRNA binding
GO:0030620 U2 snRNA binding
GO:0030623 U5 snRNA binding
GO:0097157 pre-mRNA intronic binding
Biological Process
GO:0000244 spliceosomal tri-snRNP complex assembly
GO:0000350 generation of catalytic spliceosome for second transesterification step
GO:0000387 spliceosomal snRNP assembly
GO:0000389 mRNA 3'-splice site recognition
GO:0000395 mRNA 5'-splice site recognition
GO:0000398 mRNA splicing, via spliceosome
GO:0006397 mRNA processing
GO:0008380 RNA splicing
Cellular Component
GO:0005634 nucleus
GO:0005681 spliceosomal complex
GO:0005682 U5 snRNP
GO:0005737 cytoplasm
GO:0046540 U4/U6 x U5 tri-snRNP complex
GO:0071013 catalytic step 2 spliceosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5mq0, PDBe:5mq0, PDBj:5mq0
PDBsum5mq0
PubMed28076345
UniProtP33334|PRP8_YEAST Pre-mRNA-splicing factor 8 (Gene Name=PRP8)

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