Structure of PDB 4hno Chain A Binding Site BS04

Receptor Information

>4hno Chain A (length=285) Species: 243274 (Thermotoga maritima MSB8)

MIKIGAHMPISKGFDRVPQDTVNIGGNSFQIFPHNARSWSAKLPSDEAAT
KFKREMKKHGIDWENAFCHSGYLINLASPKDDIWQKSVELLKKEVEICRK
LGIRYLNIHPGSHLGTGEEEGIDRIVRGLNEVLNNTEGVVILLENVSQKG
GNIGYKLEQLKKIRDLVDQRDRVAITYDTCHGFDSGYDITKKEGVEALLN
EIESLFGLERLKMIHLNDSKYPLGAAKDRHERIGSGFIGEEGFAVFFSFK
EIQEVPWILETPGGNEEHAEDIKKVFEIIEKFGIE

Ligand information

• Ligand ID: MN
• InChI: InChI=1S/Mn/q+2
• InChIKey: WAEMQWOKJMHJLA-UHFFFAOYSA-N
• SMILES:
  ACDLabs 10.04
  OpenEye OEToolkits 1.5.0: [Mn+2]
  CACTVS 3.341: [Mn++]
• Formula: Mn
• Name: MANGANESE (II) ION
• DrugBank: DB06757
• PDB chain: 4hno Chain A Residue 304

Receptor-Ligand Complex Structure

Structure summary

• PDB: 4hno Conserved Structural Chemistry for Incision Activity in Structurally Non-homologous Apurinic/Apyrimidinic Endonuclease APE1 and Endonuclease IV DNA Repair Enzymes.
• Resolution: 0.9194 Å
• Binding residue (original residue number in PDB): E144 D178 H215 E260
• Binding residue (residue number reindexed from 1): E144 D178 H215 E260
• Annotation score: 4

Enzymatic activity

• Catalytic site (original residue number in PDB): R37 H69 Y72 H109 E144 D178 H181 H215 D228 H230 E260
• Catalytic site (residue number reindexed from 1): R37 H69 Y72 H109 E144 D178 H181 H215 D228 H230 E260
• Enzyme Commision number: 3.1.21.2: deoxyribonuclease IV.

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003906 DNA-(apurinic or apyrimidinic site) endonuclease activity
• GO:0004519 endonuclease activity
• GO:0008081 phosphoric diester hydrolase activity
• GO:0008270 zinc ion binding
• GO:0008833 deoxyribonuclease IV (phage-T4-induced) activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006281 DNA repair
• GO:0006284 base-excision repair

External links

• PDB: RCSB:4hno, PDBe:4hno, PDBj:4hno
• PDBsum: 4hno
• PubMed: 23355472
• UniProt: Q9WYJ7|END4_THEMA Probable endonuclease 4 (Gene Name=nfo)