Structure of PDB 5gad Chain i Binding Site BS03

Receptor Information

>5gad Chain i (length=450) Species: 562 (Escherichia coli)

NLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVALPVVREFIN
RVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAVV
LMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAE
QVGVDFFPSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMD
EIKQVHASINPVETLFVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGD
ARGGAALSIRHITGKPIKFLGVGEKTEALEPFHPDRIASRILGMPPPPPP
PPPPPPPPPPPPPPPPPPPPPPPPGFDLNDFLEQLRQMKNMGGPPPPPPP
PPPPPPPPPPPPPPPDDKVLVRMEAIINSMTMKERAKPEIIKGSRKRRIA
AGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGPPPPPPPPPPPPPPPPPPP

Ligand information

>5gad Chain k (length=18) Species: 562 (Escherichia coli)

MKQSTLALLLLLLLLTPV

Receptor-Ligand Complex Structure

Structure summary

• PDB: 5gad Structures of the E. coli translating ribosome with SRP and its receptor and with the translocon.
• Resolution: 3.7 Å
• Binding residue (original residue number in PDB): X313 X317 Q337 M341 X354 Q419 M423 M426
• Binding residue (residue number reindexed from 1): X310 X314 Q334 M338 X351 Q416 M420 M423

Enzymatic activity

• Enzyme Commision number: 3.6.5.4: signal-recognition-particle GTPase.

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003924 GTPase activity
• GO:0005515 protein binding
• GO:0005525 GTP binding
• GO:0008312 7S RNA binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity

Biological Process

• GO:0006612 protein targeting to membrane
• GO:0006614 SRP-dependent cotranslational protein targeting to membrane

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0048500 signal recognition particle
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5gad, PDBe:5gad, PDBj:5gad
• PDBsum: 5gad
• PubMed: 26804923
• UniProt: P0AGD7|SRP54_ECOLI Signal recognition particle protein (Gene Name=ffh)